Intranasal inoculation with an adenovirus vaccine encoding ten repeats of Aβ3-10 induces Th2 immune response against amyloid-β in wild-type mouse.

Abstract

To develop a safe and efficient vaccine for the treatment of Alzheimer's disease, we constructed a novel adenovirus vaccine encoding ten repeats of Aβ3-10 and CpG motif as an adjuvant and investigated the immune response in BALB/c mouse after intranasal inoculation with this vaccine. The Ad-10×Aβ3-10-CpG induces an IgG1 predominant humoral response and production of IL-4 and IL-10 in splenocytes in vitro, indicating a Th2-polarized immune response. Stimulation of splenocytes with Aβ3-10 peptide induces robust proliferation but not with full-length Aβ42 peptide, demonstrating that Ad-10×Aβ3-10-CpG does not induces Aβ42 specific T cell immune response. The findings raise the possibility that the adenovirus vaccine Ad-10×Aβ3-10-CpG could be a safe and effective alternative for immunotherapy in Alzheimer's disease.

• Authors: Yu Li, Ying Ma, Li-Xia Zong, Xiao-Na Xing, Sha Sha, Yun-Peng Cao
• Journal: Neuroscience letters (Neurosci Lett) Vol. 505 Issue 2 Pg. 128-33 (Nov 14 2011) ISSN: 1872-7972 [Electronic] Ireland
• PMID: 22005583 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Crown Copyright © 2011. Published by Elsevier Ireland Ltd. All rights reserved.

Chemical References

• Amyloid beta-Peptides
• IL10 protein, mouse
• Viral Vaccines
• Interleukin-10
• Interleukin-4

Topics

• Adenoviridae (genetics, immunology)
• Administration, Intranasal
• Alzheimer Disease (immunology, pathology, prevention & control)
• Amyloid beta-Peptides (administration & dosage, genetics, immunology)
• Animals
• Cell Polarity (genetics, immunology)
• Cells, Cultured
• Genetic Vectors (administration & dosage, immunology)
• Humans
• Immunity, Cellular (genetics)
• Interleukin-10 (biosynthesis)
• Interleukin-4 (biosynthesis)
• Male
• Mice
• Mice, Inbred BALB C
• Mice, Transgenic
• Repetitive Sequences, Amino Acid (genetics, immunology)
• Spleen (immunology, metabolism)
• Th2 Cells (immunology, virology)
• Viral Vaccines (administration & dosage, genetics, immunology)