Abstract |
Tubeimoside-1 is a triterpenoid saponin extracted from the traditional Chinese herb Bolbostemma paniculatum (Maxim.) Franquet (Cucurbitaceae). We investigated the cytotoxic effect and apoptosis mechanism of tubeimoside-1. Tubeimoside-1 was cytotoxic in seven human cancer cell lines, with HepG2 the most sensitive. Tubeimoside-1 induced apoptosis of HepG2 cells dose and time dependently. Both the extrinsic and intrinsic pathways were triggered by tubeimoside-1. Caspase-3, -8 and -9 were activated and the expression of Fas, Fas ligand, Bcl-2, Bak and Bax was regulated. Moreover, tubeimoside-1 induced accumulation of reactive oxygen species and arrested cell cycle at the G(2)/M phase, thus contributing to apoptosis, through signaling regulation by tumor necrosis factor α, nuclear factor κB (NF-κB), Jun N-terminal kinase (JNK) and p53. We provide further insight into the tubeimoside-1 cytotoxic effect for antitumor chemotherapeutic treatment.
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Authors | Yan Yin, Wei Chen, Changyan Tang, Hanjing Ding, Jongchol Jang, Meizhi Weng, Yongjun Cai, Guolin Zou |
Journal | Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
(Food Chem Toxicol)
Vol. 49
Issue 12
Pg. 3046-54
(Dec 2011)
ISSN: 1873-6351 [Electronic] England |
PMID | 22005259
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2011 Elsevier Ltd. All rights reserved. |
Chemical References |
- Drugs, Chinese Herbal
- Fas Ligand Protein
- NF-kappa B
- Reactive Oxygen Species
- Saponins
- TP53 protein, human
- Triterpenes
- Tumor Necrosis Factor-alpha
- Tumor Suppressor Protein p53
- bcl-2-Associated X Protein
- tubeimoside I
- JNK Mitogen-Activated Protein Kinases
- CASP3 protein, human
- CASP8 protein, human
- CASP9 protein, human
- Caspase 3
- Caspase 8
- Caspase 9
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Topics |
- Apoptosis
(drug effects)
- Caspase 3
(genetics, metabolism)
- Caspase 8
(genetics, metabolism)
- Caspase 9
(genetics, metabolism)
- Cucurbitaceae
(chemistry)
- Drugs, Chinese Herbal
(pharmacology)
- Fas Ligand Protein
(genetics, metabolism)
- G2 Phase Cell Cycle Checkpoints
(drug effects)
- Gene Expression Regulation
- Hep G2 Cells
- Humans
- JNK Mitogen-Activated Protein Kinases
(genetics, metabolism)
- NF-kappa B
(genetics, metabolism)
- Oxidative Stress
(drug effects)
- Reactive Oxygen Species
(metabolism)
- Saponins
(pharmacology)
- Signal Transduction
- Triterpenes
(pharmacology)
- Tumor Necrosis Factor-alpha
(genetics, metabolism)
- Tumor Suppressor Protein p53
(genetics, metabolism)
- bcl-2-Associated X Protein
(genetics, metabolism)
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