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Enumeration of viable Enterococcus faecalis, a predominant apical periodontitis pathogen, using propidium monoazide and quantitative real-time polymerase chain reaction.

Abstract
To discriminate between viable and non-viable Enterococcus faecalis, the predominant pathogen in apical periodontitis, a real-time PCR method combined with propidium monoazide (PMA) was developed and evaluated. PMA had no antimicrobial effect on E. faecalis cells and permitted enumeration of both viable and non-viable cells. Therefore, E. faecalis cells from the root canals of nine patients with apical periodontitis were analyzed to evaluate the diagnostic usefulness of this approach. Viable and non-viable E. faecalis cells were successfully discriminated in these clinical specimens. A real-time PCR assay combined with PMA will contribute to the precise diagnosis of apical periodontitis.
AuthorsYusuke Takahashi, Akihiro Yoshida, Masato Nagayoshi, Chiaki Kitamura, Tatsuji Nishihara, Shuji Awano, Toshihiro Ansai
JournalMicrobiology and immunology (Microbiol Immunol) Vol. 55 Issue 12 Pg. 889-92 (Dec 2011) ISSN: 1348-0421 [Electronic] Australia
PMID22003816 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Copyright© 2011 The Societies and Blackwell Publishing Asia Pty Ltd.
Chemical References
  • Azides
  • propidium monoazide
  • Propidium
Topics
  • Azides (metabolism)
  • Colony Count, Microbial
  • Dental Pulp Cavity (microbiology)
  • Enterococcus faecalis (genetics, growth & development, isolation & purification)
  • Genes, Bacterial (genetics)
  • Gram-Positive Bacterial Infections (microbiology)
  • Humans
  • Microbial Viability
  • Periapical Periodontitis (microbiology)
  • Propidium (analogs & derivatives, metabolism)
  • Real-Time Polymerase Chain Reaction (methods)

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