Abstract |
To discriminate between viable and non-viable Enterococcus faecalis, the predominant pathogen in apical periodontitis, a real-time PCR method combined with propidium monoazide (PMA) was developed and evaluated. PMA had no antimicrobial effect on E. faecalis cells and permitted enumeration of both viable and non-viable cells. Therefore, E. faecalis cells from the root canals of nine patients with apical periodontitis were analyzed to evaluate the diagnostic usefulness of this approach. Viable and non-viable E. faecalis cells were successfully discriminated in these clinical specimens. A real-time PCR assay combined with PMA will contribute to the precise diagnosis of apical periodontitis.
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Authors | Yusuke Takahashi, Akihiro Yoshida, Masato Nagayoshi, Chiaki Kitamura, Tatsuji Nishihara, Shuji Awano, Toshihiro Ansai |
Journal | Microbiology and immunology
(Microbiol Immunol)
Vol. 55
Issue 12
Pg. 889-92
(Dec 2011)
ISSN: 1348-0421 [Electronic] Australia |
PMID | 22003816
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2011 The Societies and Blackwell Publishing Asia Pty Ltd. |
Chemical References |
- Azides
- propidium monoazide
- Propidium
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Topics |
- Azides
(metabolism)
- Colony Count, Microbial
- Dental Pulp Cavity
(microbiology)
- Enterococcus faecalis
(genetics, growth & development, isolation & purification)
- Genes, Bacterial
(genetics)
- Gram-Positive Bacterial Infections
(microbiology)
- Humans
- Microbial Viability
- Periapical Periodontitis
(microbiology)
- Propidium
(analogs & derivatives, metabolism)
- Real-Time Polymerase Chain Reaction
(methods)
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