The neonatal Fc receptor (FcRn) is not required for IVIg or anti-CD44 monoclonal antibody-mediated amelioration of murine immune thrombocytopenia.

Abstract

To definitively determine whether the neonatal Fc receptor (FcRn) is required for the acute amelioration of immune thrombocytopenia (ITP) by IVIg, we used FcRn-deficient mice in a murine ITP model. Mice injected with antiplatelet antibody in the presence or absence of IVIg displayed no difference in platelet-associated IgG between FcRn deficient versus C57BL/6 mice. FcRn-deficient mice treated with high-dose (2 g/kg) IVIg or a low-dose (2 mg/kg) of an IVIg-mimetic CD44 antibody were, however, protected from thrombocytopenia to an equivalent extent as wild-type mice. To verify and substantiate the results found with FcRn-deficient mice, we used β(2)-microglobulin-deficient mice (which do not express functional FcRn) and found that IVIg or CD44 antibody also protected them from thrombocytopenia. These data suggest that for both high-dose IVIg as well as low-dose CD44 antibody treatment in an acute ITP model, FcRn expression is neither necessary nor required.

• Authors: Andrew R Crow, Sara J Suppa, Xi Chen, Patrick J Mott, Alan H Lazarus
• Journal: Blood (Blood) Vol. 118 Issue 24 Pg. 6403-6 (Dec 08 2011) ISSN: 1528-0020 [Electronic] United States
• PMID: 22001393 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Monoclonal
• Autoantibodies
• Cd44 protein, mouse
• Histocompatibility Antigens Class I
• Hyaluronan Receptors
• Immunoglobulins, Intravenous
• Receptors, Fc
• beta 2-Microglobulin
• Fc receptor, neonatal

Topics

• Animals
• Antibodies, Monoclonal (therapeutic use)
• Autoantibodies (therapeutic use)
• Disease Models, Animal
• Histocompatibility Antigens Class I (genetics, physiology)
• Humans
• Hyaluronan Receptors (chemistry)
• Immunoglobulins, Intravenous (therapeutic use)
• Mice
• Mice, Inbred C57BL
• Mice, Knockout
• Mice, Transgenic
• Platelet Count
• Purpura, Thrombocytopenic, Idiopathic (blood, immunology, prevention & control)
• Receptors, Fc (genetics, physiology)
• beta 2-Microglobulin (genetics, physiology)