Enzyme-linked immunosorbent assay for immunoglobulin M specific antibody for the diagnosis of melioidosis.

Indirect hemagglutination (IHA) is commonly used for serodiagnosis of melioidosis. However, in endemic areas, high background titers in normal populations and occasional low titers in patients with septicemic melioidosis prompted a search for a more sensitive and more specific method of serodiagnosis. An indirect fluorescent-antibody test for immunoglobulin M (IgM) specific antibody to Pseudomonas pseudomallei was more sensitive and more specific, but fluorescence microscopes are rarely available in the endemic areas. An enzyme-linked immunosorbent assay (ELISA) for IgM antibody is an attractive alternative. An indirect ELISA for IgM antibody (IgM ELISA) and an IgM antibody capture ELISA for melioidosis were developed. Both tests, together with IHA, were evaluated for 153 serum specimens from blood donors and 16 serum specimens from 16 melioidosis patients. It was found that IHA, the IgM ELISA, and the IgM antibody capture ELISA had sensitivities of 88, 88, and 75%, respectively, with specificities of 97.4, 92.2, and 91.5%, respectively. When IHA was combined with IgM ELISA, a sensitivity of 100% and a specificity of 95.4% were obtained. The IgM ELISA and IHA should be used in combination for serodiagnosis of melioidosis.
AuthorsM Kunakorn, P Boonma, K Khupulsup, B Petchclai
JournalJournal of clinical microbiology (J Clin Microbiol) Vol. 28 Issue 6 Pg. 1249-53 (Jun 1990) ISSN: 0095-1137 [Print] UNITED STATES
PMID2199494 (Publication Type: Journal Article)
Chemical References
  • Antibodies, Bacterial
  • Immunoglobulin M
  • Antibodies, Bacterial (immunology)
  • Enzyme-Linked Immunosorbent Assay
  • Hemagglutination Tests
  • Humans
  • Immunoglobulin M (analysis)
  • Melioidosis (diagnosis)
  • Predictive Value of Tests
  • Pseudomonas (immunology)
  • Sensitivity and Specificity

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!

Choose Username:
Verify Password: