Black tea extract prevents lipopolysaccharide-induced NF-κB signaling and attenuates dextran sulfate sodium-induced experimental colitis.

Abstract

BACKGROUND:

Black tea has been shown to elicit anti-oxidant, anti-carcinogenic, anti-inflammatory and anti-mutagenic properties. In this study, we investigated the impact of black tea extract (BTE) on lipopolysaccharide (LPS)-induced NF-κB signaling in bone marrow derived-macrophages (BMM) and determined the therapeutic efficacy of this extract on colon inflammation.

METHODS:

The effect of BTE on LPS-induced NF-κB signaling and pro-inflammatory gene expression was evaluated by RT-PCR, Western blotting, immunofluorescence and electrophoretic mobility shift assay (EMSA). The in vivo efficacy of BTE was assessed in mice with 3% dextran sulfate sodium (DSS)-induced colitis. The severity of colitis was measured by weight loss, colon length and histologic scores.

RESULTS:

LPS-induced IL-12p40, IL-23p19, IL-6 and IL-1β mRNA expressions were inhibited by BTE. LPS-induced IκBα phosphorylation/degradation and nuclear translocation of NF-κB/p65 were blocked by BTE. BTE treatment blocked LPS-induced DNA-binding activity of NF-κB. BTE-fed, DSS-exposed mice showed the less weight loss, longer colon length and lower histologic score compared to control diet-fed, DSS-exposed mice. DSS-induced IκBα phosphorylation/degradation and phosphorylation of NF-κB/p65 were blocked by BTE. An increase of cleaved caspase-3 and poly (ADP-ribose) polymerase (PARP) in DSS-exposed mice was blocked by BTE.

CONCLUSIONS:

These results indicate that BTE attenuates colon inflammation through the blockage of NF-κB signaling and apoptosis in DSS-induced experimental colitis model.

• Authors: Young-A Song, Young-Lan Park, Kyu-Yeol Kim, Cho-Yun Chung, Gi-Hoon Lee, Dae-Ho Cho, Ho-Seok Ki, Kang-Jin Park, Sung-Bum Cho, Wan-Sik Lee, Nacksung Kim, Bong-Whan Ahn, Young-Eun Joo
• Journal: BMC complementary and alternative medicine (BMC Complement Altern Med) Vol. 11 Pg. 91 (Oct 11 2011) ISSN: 1472-6882 [Electronic] England
• PMID: 21989142 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cytokines
• Lipopolysaccharides
• NF-kappa B
• Plant Extracts
• Dextran Sulfate

Topics

• Animals
• Camellia sinensis (chemistry)
• Cells, Cultured
• Colitis (chemically induced, drug therapy, genetics, immunology)
• Cytokines (genetics, immunology)
• Dextran Sulfate (adverse effects)
• Disease Models, Animal
• Down-Regulation (drug effects)
• Gene Expression (drug effects)
• Humans
• Lipopolysaccharides (immunology)
• Macrophages (drug effects, immunology)
• Mice
• Mice, Inbred C57BL
• NF-kappa B (immunology)
• Plant Extracts (pharmacology)
• Signal Transduction (drug effects)