HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Composition and localization of Treponema denticola outer membrane complexes.

Abstract
The Treponema denticola outer membrane lipoprotein-protease complex (dentilisin) contributes to periodontal disease by degrading extracellular matrix components and disrupting intercellular host signaling pathways. We recently demonstrated that prcB, located upstream of and cotranscribed with prcA and prtP, encodes a 22-kDa lipoprotein that interacts with PrtP and is required for its activity. Here we further characterize products of the protease locus and their roles in expression, formation, and localization of outer membrane complexes. PrcB migrates in native gels as part of a >400-kDa complex that includes PrtP and PrcA, as well as the major outer sheath protein Msp. PrcB is detectable as a minor constituent of the purified active protease complex, which was previously reported to consist of only PrtP and auxiliary polypeptides PrcA1 and PrcA2. Though it lacks the canonical ribosome binding site present upstream of both prcA and prtP, PrcB is present at levels similar to those of PrtP in whole-cell extracts. Immunofluorescence microscopy demonstrated cell surface exposure of the mature forms of PrtP, PrcA1, PrcB, and Msp. The 16-kDa N-terminal acylated fragment of PrtP (predicted to be released during activation of PrtP) was present in cell extracts but was detected neither in the purified active protease complex nor on the cell surface. PrcA2, detectable on the surface of Msp-deficient cells but not that of wild-type cells, coimmunoprecipitated with Msp. Our results indicate that PrcB is a component of the outer membrane lipoprotein protease complex and that Msp and PrcA2 interaction may mediate formation of a very-high-molecular-weight outer membrane complex.
AuthorsValentina Godovikova, M Paula Goetting-Minesky, J Christopher Fenno
JournalInfection and immunity (Infect Immun) Vol. 79 Issue 12 Pg. 4868-75 (Dec 2011) ISSN: 1098-5522 [Electronic] United States
PMID21986628 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)
Chemical References
  • Bacterial Outer Membrane Proteins
  • Bacterial Proteins
  • Porins
  • PrcA protein, bacteria
  • Virulence Factors
  • major outer sheath protein, Treponema
  • Peptide Hydrolases
  • Subtilisins
  • dentilisin
  • Chymotrypsin
Topics
  • Bacterial Outer Membrane Proteins (genetics, metabolism)
  • Bacterial Proteins (genetics, metabolism)
  • Blotting, Western
  • Chymotrypsin (genetics, metabolism)
  • Electrophoresis, Polyacrylamide Gel
  • Gene Expression Regulation, Bacterial (physiology)
  • Peptide Hydrolases
  • Porins (genetics, metabolism)
  • Protein Transport
  • Subtilisins (genetics, metabolism)
  • Treponema denticola (cytology, metabolism)
  • Virulence Factors (genetics, metabolism)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: