Abstract |
Dibromoacetonitrile (DBAN) is water disinfectant by-product. Its broad-spectrum toxicity in different test systems in vivo and in vitro has been reported. However, there is a scanty of information regarding dibromoacetonitrile hepatotoxicity. Therefore, this study aimed to investigate the possible mechanisms for dibromoacetonitrile-induced tumor initiation in rat liver cells. Dibromoacetonitrile was orally administered to rats as an acute (60 mg/kg) and fractionated (7.5mg/kg) doses, weekly twice for 4 weeks and once weekly for 8 weeks. Significant increase in malondialdehyde level (approximately 7-, 6- and 4-folds) and extensive depletion in total antioxidant capacity were detected following acute and fractionated doses respectively. Alanine aminotransferase (about 2- and 1-folds) and aspartate aminotransferase (3- and 2-folds) were significantly increased after acute dose and fractionated doses for 4 weeks. Also, these doses of dibromoacetonitrile produced high levels of DNA fragmentation, micronucleated polychromatic erythrocytes and changes in the expression of hepatocyte growth factor gene and proto-oncogenes (c-met and c-myc) in liver tissues. Ability of dibromoacetonitrile to induce DNA damage and alterations in the expression of tumor-initiating genes was suggested to be due to hepatotoxicity, oxidative stress and disturbance in the oxidant/ antioxidant status of rat liver.
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Authors | Naglaa Assaf, Neveen A Salem, Wagdy K B Khalil, Hanaa H Ahmed |
Journal | Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
(Food Chem Toxicol)
Vol. 49
Issue 12
Pg. 3055-62
(Dec 2011)
ISSN: 1873-6351 [Electronic] England |
PMID | 21986297
(Publication Type: Journal Article)
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Copyright | Copyright © 2011 Elsevier Ltd. All rights reserved. |
Chemical References |
- Acetonitriles
- Antioxidants
- Carcinogens
- Malondialdehyde
- Aspartate Aminotransferases
- Alanine Transaminase
- dibromoacetonitrile
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Topics |
- Acetonitriles
(toxicity)
- Alanine Transaminase
(analysis, metabolism)
- Animals
- Antioxidants
(metabolism)
- Apoptosis
(drug effects)
- Aspartate Aminotransferases
(analysis, metabolism)
- Carcinogens
(toxicity)
- DNA Damage
(drug effects)
- DNA Fragmentation
(drug effects)
- Dose-Response Relationship, Drug
- Erythrocytes
(drug effects, metabolism)
- Liver
(drug effects, pathology)
- Male
- Malondialdehyde
(analysis)
- Oxidative Stress
(drug effects)
- Rats
- Rats, Sprague-Dawley
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