Analysis of stromal cells in osteofibrous dysplasia and adamantinoma of long bones.

Abstract

Adamantinoma of long bones and osteofibrous dysplasia are rare, osteolytic primary bone tumours of uncertain origin containing areas of fibrous and fibro-osseous proliferation. We investigated the nature of the stromal cells in adamantinoma of long bones and osteofibrous dysplasia, and determined cellular and molecular mechanisms of osteolysis in these tumours. Cell culture, molecular (RT-PCR, western blot) and immunohistochemical studies on cases of adamantinoma of long bones and of osteofibrous dysplasia were undertaken to determine the expression of epithelial, osteoblast and osteoclast markers. Ultrastructural and immunophenotypic studies on cultured adamantinoma and osteofibrous dysplasia stromal cells showed that these cells were mainly fibroblast-like with few cells expressing epithelial markers. Osteofibrous dysplasia but not adamantinoma cells expressed alkaline phosphatase. Both osteofibrous dysplasia and adamantinoma cells expressed the ostoclastogenic factors M-CSF and RANKL. Adamantinoma and osteofibrous dysplasia cells also expressed messenger RNA for osteocalcin, osteonectin, osteopontin, osterix and collagen type 1. Adamantinoma and osteofibrous dysplasia cells cultured alone on dentine slices were not capable of lacunar resorption, but in co-cultures with monocytes induced formation of osteoclast-like cells was observered. Cultured osteofibrous dysplasia and adamantinoma stromal cells show similar ultrastructural and immunophenotypic characteristics, and differentially express osteoblast markers. Promotion of osteoclastogenesis by stromal cells may contribute to osteolysis in adamantinoma of long bones and osteofibrous dysplasia.

• Authors: Richard M Taylor, Takeshi G Kashima, David J Ferguson, Károly Szuhai, Pancras C Hogendoorn, Nicholas A Athanasou
• Journal: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc (Mod Pathol) Vol. 25 Issue 1 Pg. 56-64 (Jan 2012) ISSN: 1530-0285 [Electronic] United States
• PMID: 21983933 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers, Tumor

Topics

• Adamantinoma (genetics, immunology, metabolism, pathology, ultrastructure)
• Adolescent
• Biomarkers, Tumor (metabolism)
• Blotting, Western
• Cells, Cultured
• Child
• Female
• Fibrous Dysplasia of Bone (genetics, immunology, metabolism, pathology)
• Humans
• Immunohistochemistry
• Immunophenotyping
• Middle Aged
• Polymerase Chain Reaction
• Stromal Cells (metabolism, pathology, ultrastructure)
• Tibia (immunology, metabolism, pathology, ultrastructure)
• Young Adult