Identification and phylogenetic analysis of Japanese Macaque Babesia-1 (JM-1) detected from a Japanese Macaque (Macaca fuscata fuscata).

Abstract

We demonstrate here the identification and phylogenetic characterization of Babesia microti (B. microti)-like parasite detected from a splenectomized Japanese macaque (Macaca fuscata fuscata) at a facility for laboratory animal science. On Day 133 after splenectomy, intra-erythrocytic parasites were found on light microscopic examination, and the level of parasitemia reached 0.3% on blood smear. Molecular characterization of the parasite using nested-polymerization chain reactions targeting the 18S rRNA, β-tubulin, and subunit 7 (eta) of the chaperonin-containing t-complex polypeptide 1 (CCT7) genes were identified as a B. microti-like parasite, designated the Japanese Macaque Babesia-1 (JM-1).

• Authors: Haruyuki Hirata, Satoru Kawai, Mari Maeda, Michio Jinnai, Kohei Fujisawa, Yuko Katakai, Kenji Hikosaka, Kazuyuki Tanabe, Yasuhiro Yasutomi, Chiaki Ishihara
• Journal: The American journal of tropical medicine and hygiene (Am J Trop Med Hyg) Vol. 85 Issue 4 Pg. 635-8 (Oct 2011) ISSN: 1476-1645 [Electronic] United States
• PMID: 21976563 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA Primers

Topics

• Animals
• Babesia microti (genetics, isolation & purification)
• Base Sequence
• DNA Primers
• Female
• Macaca
• Phylogeny
• Polymerase Chain Reaction
• Splenectomy