Nonmuscle
myosin IIA (
myosin IIA) is a force-producing
protein involved in the process of cell migration. Its expression has been considered as a bad prognostic
indicator in stage I
lung adenocarcinoma. However, the expression and clinical significance of
myosin IIA in
esophageal cancer has not been explored. In this study, we investigate the expression level of
myosin IIA in 50 esophageal squamous
cancer and 30 adjacent normal esophageal tissues by immunohistochemical staining and correlated its expression with clinicopathological features.
Myosin IIA was expressed in all esophageal squamous
cancer tissues (100%) and 8 of 30 adjacent normal tissues (26.7%, P = 0.000). In
cancer tissues, elevated
myosin IIA expression level was significantly correlated with increasing metastatic lymph nodes, poorer
cancer differentiation, and advanced
tumor stage. Further univariate analysis suggested that strong
myosin IIA expression was associated with a significantly shorter overall survival (P = 0.021). In addition, MYH9
SiRNA was transfected into esophageal squamous
cancer cell line (KYSE-510) to study the role of
myosin IIA in cell migration.
SiRNA-mediated depletion of
myosin IIA in KYSE-510 cells significantly increased cell-matrix adhesion and attenuated cell migration ability (P = 0.000). In conclusion, these findings indicate that overexpression of
myosin IIA may contribute to the progression and poor prognosis of esophageal squamous
cancer, and this effect may be associated with increased
cancer cell migration.