An unconventional pathway of mRNA cap formation by vesiculoviruses.

Abstract

mRNAs of vesicular stomatitis virus (VSV), a prototype of nonsegmented negative strand (NNS) RNA viruses (e.g., rabies, measles, mumps, Ebola, and Borna disease viruses), possess the 5'-terminal cap structure identical to that of eukaryotic mRNAs, but the mechanism of mRNA cap formation is distinctly different from the latter. The elucidation of the unconventional capping of VSV mRNA remained elusive for three decades since the discovery of the cap structure in some viral and eukaryotic mRNAs in 1975. Only recently our biochemical studies revealed an unexpected strategy employed by vesiculoviruses (VSV and Chandipura virus, an emerging arbovirus) to generate the cap structure. This article summarizes the historical and current research that led to the discovery of the novel vesiculoviral mRNA capping reaction.

• Authors: Tomoaki Ogino, Amiya K Banerjee
• Journal: Virus research (Virus Res) Vol. 162 Issue 1-2 Pg. 100-9 (Dec 2011) ISSN: 1872-7492 [Electronic] Netherlands
• PMID: 21945214 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Review)
• Copyright: Copyright © 2011 Elsevier B.V. All rights reserved.

Chemical References

• RNA Caps
• RNA, Viral
• Viral Proteins
• Nucleotidyltransferases
• guanylyltransferase
• Polyribonucleotide Nucleotidyltransferase

Topics

• Amino Acid Sequence
• Humans
• Molecular Sequence Data
• Nucleotidyltransferases (chemistry, genetics, metabolism)
• Polyribonucleotide Nucleotidyltransferase (chemistry, genetics, metabolism)
• RNA Caps (chemistry, genetics, metabolism)
• RNA, Viral (genetics, metabolism)
• Sequence Alignment
• Vesicular Stomatitis (virology)
• Vesicular stomatitis Indiana virus (chemistry, genetics, metabolism)
• Vesiculovirus (chemistry, genetics, metabolism)
• Viral Proteins (chemistry, genetics, metabolism)