Abstract |
MicroRNAs ( miRNA, miR) have been reported as cancer biomarkers that regulate tumor suppressor genes. Hence, simultaneous detecting and inhibiting of miRNA function will be useful as a cancer theragnostics probe to minimize side effects and invasiveness. In this study, we developed a cancer-targeting therangostics probe in a single system using an AS1411 aptamer - and miRNA-221 molecular beacon (miR-221 MB)-conjugated magnetic fluorescence (MF) nanoparticle (MFAS miR-221 MB) to simultaneously target to cancer tissue, image intracellularly expressed miRNA-221 and treat miRNA-221-involved carcinogenesis. AS1411 aptamer-conjugated MF (MFAS) nanoparticles displayed a great selectivity and delivery into various cancer cell lines. The miR-221 MB detached from the MFAS miR-221 MB in the cytoplasm of C6 cells clearly imaged miRNA-221 biogenesis and simultaneously resulted in antitumor therapeutic effects by inhibiting miRNA function, indicating a successful astrocytoma-targeting theragnostics. MFAS miRNA MB can be easily applied to other cancers by simply changing a targeted miRNA highly expressed in cancers.
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Authors | Jin Kyeoung Kim, Kyung-Ju Choi, Minhyung Lee, Mi-hee Jo, Soonhag Kim |
Journal | Biomaterials
(Biomaterials)
Vol. 33
Issue 1
Pg. 207-17
(Jan 2012)
ISSN: 1878-5905 [Electronic] Netherlands |
PMID | 21944470
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2011 Elsevier Ltd. All rights reserved. |
Chemical References |
- AGRO 100
- Aptamers, Nucleotide
- MicroRNAs
- Oligodeoxyribonucleotides
- Phosphoproteins
- RNA-Binding Proteins
- nucleolin
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Topics |
- Animals
- Aptamers, Nucleotide
- Caco-2 Cells
- Cell Line, Tumor
- Cell Survival
(drug effects)
- HeLa Cells
- Humans
- Mice
- Mice, Nude
- MicroRNAs
(chemistry)
- Microscopy, Confocal
- Nanoparticles
(chemistry, therapeutic use)
- Neoplasms
(drug therapy)
- Oligodeoxyribonucleotides
(chemistry, therapeutic use)
- Phosphoproteins
(chemistry)
- RNA-Binding Proteins
(chemistry)
- Real-Time Polymerase Chain Reaction
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