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Molecular imaging of a cancer-targeting theragnostics probe using a nucleolin aptamer- and microRNA-221 molecular beacon-conjugated nanoparticle.

Abstract
MicroRNAs (miRNA, miR) have been reported as cancer biomarkers that regulate tumor suppressor genes. Hence, simultaneous detecting and inhibiting of miRNA function will be useful as a cancer theragnostics probe to minimize side effects and invasiveness. In this study, we developed a cancer-targeting therangostics probe in a single system using an AS1411 aptamer - and miRNA-221 molecular beacon (miR-221 MB)-conjugated magnetic fluorescence (MF) nanoparticle (MFAS miR-221 MB) to simultaneously target to cancer tissue, image intracellularly expressed miRNA-221 and treat miRNA-221-involved carcinogenesis. AS1411 aptamer-conjugated MF (MFAS) nanoparticles displayed a great selectivity and delivery into various cancer cell lines. The miR-221 MB detached from the MFAS miR-221 MB in the cytoplasm of C6 cells clearly imaged miRNA-221 biogenesis and simultaneously resulted in antitumor therapeutic effects by inhibiting miRNA function, indicating a successful astrocytoma-targeting theragnostics. MFAS miRNA MB can be easily applied to other cancers by simply changing a targeted miRNA highly expressed in cancers.
AuthorsJin Kyeoung Kim, Kyung-Ju Choi, Minhyung Lee, Mi-hee Jo, Soonhag Kim
JournalBiomaterials (Biomaterials) Vol. 33 Issue 1 Pg. 207-17 (Jan 2012) ISSN: 1878-5905 [Electronic] Netherlands
PMID21944470 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2011 Elsevier Ltd. All rights reserved.
Chemical References
  • AGRO 100
  • Aptamers, Nucleotide
  • MicroRNAs
  • Oligodeoxyribonucleotides
  • Phosphoproteins
  • RNA-Binding Proteins
  • nucleolin
Topics
  • Animals
  • Aptamers, Nucleotide
  • Caco-2 Cells
  • Cell Line, Tumor
  • Cell Survival (drug effects)
  • HeLa Cells
  • Humans
  • Mice
  • Mice, Nude
  • MicroRNAs (chemistry)
  • Microscopy, Confocal
  • Nanoparticles (chemistry, therapeutic use)
  • Neoplasms (drug therapy)
  • Oligodeoxyribonucleotides (chemistry, therapeutic use)
  • Phosphoproteins (chemistry)
  • RNA-Binding Proteins (chemistry)
  • Real-Time Polymerase Chain Reaction

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