In vivo imaging of epileptic activity using 2-NBDG, a fluorescent deoxyglucose analog.

Abstract

Accurately locating epileptic foci has great importance in advancing the treatment of epilepsy. In this study, epileptic seizures were first induced by intracortical injection of 4-aminopyridine in rats. A fluorescent deoxyglucose substitute, 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG), was then continuously injected via the tail vein. Brain glucose metabolism was subsequently monitored by fluorescence imaging of 2-NBDG. The initial uptake rate of 2-NBDG at the injection site of 4-aminopyridine significantly exceeded that of the control injection site, which indicated local hypermetabolism induced by seizures. Our results show that 2-NBDG can be used for localizing epileptic foci.

• Authors: Vassiliy Tsytsarev, Konstantin I Maslov, Junjie Yao, Archana R Parameswar, Alexei V Demchenko, Lihong V Wang
• Journal: Journal of neuroscience methods (J Neurosci Methods) Vol. 203 Issue 1 Pg. 136-40 (Jan 15 2012) ISSN: 1872-678X [Electronic] Netherlands
• PMID: 21939688 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)
• Copyright: Copyright © 2011 Elsevier B.V. All rights reserved.

Chemical References

• Convulsants
• Fluorescent Dyes
• Deoxyglucose
• 4-Aminopyridine
• 4-Chloro-7-nitrobenzofurazan
• Glucose
• 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose

Topics

• 4-Aminopyridine (toxicity)
• 4-Chloro-7-nitrobenzofurazan (analogs & derivatives)
• Animals
• Brain (metabolism)
• Convulsants (toxicity)
• Deoxyglucose (analogs & derivatives)
• Epilepsy (chemically induced, metabolism)
• Fluorescent Dyes
• Glucose (metabolism)
• Image Processing, Computer-Assisted
• Male
• Rats
• Rats, Sprague-Dawley