Abstract | PURPOSE: METHODS: Twenty-one guinea pigs were randomly assigned to one of three groups of seven animals each: no-PVR/saline group (no PVR/intravitreal saline-injected group), PVR/saline group ( dispase-induced PVR group, treated with control injections of intravitreal saline), and PVR/ tacrolimus group (treatment group, dispase-induced PVR group treated with intravitreal tacrolimus injections). At the end of the experiment, eyes were enucleated and the identification of the stages of PVR was carried out. While a halves of the enucleated globes were evaluated histopathologically for PVR formation, the retinas of the other halves of globes were used for the preparation of retinal homogenates. The transforming growth factor-β, platelet-derived growth factor, and fibroblast growth factor levels in homogenized retina tissues were measured by the enzyme-linked immunosorbent assay method. RESULTS: CONCLUSION: This study suggests that intravitreal tacrolimus application may suppress PVR development and that tacrolimus may merit investigation for the prophylaxis of PVR.
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Authors | Burak Turgut, Fatma Uyar, Bilal Ustundag, Ulku Celiker, Nusret Akpolat, Tamer Demir |
Journal | Retina (Philadelphia, Pa.)
(Retina)
Vol. 32
Issue 2
Pg. 232-41
(Feb 2012)
ISSN: 1539-2864 [Electronic] United States |
PMID | 21934553
(Publication Type: Journal Article)
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Chemical References |
- Immunosuppressive Agents
- Intercellular Signaling Peptides and Proteins
- Platelet-Derived Growth Factor
- Transforming Growth Factor beta
- Fibroblast Growth Factor 2
- Tacrolimus
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Topics |
- Animals
- Disease Models, Animal
- Enzyme-Linked Immunosorbent Assay
- Fibroblast Growth Factor 2
(metabolism)
- Guinea Pigs
- Immunosuppressive Agents
(administration & dosage)
- Intercellular Signaling Peptides and Proteins
(metabolism)
- Intravitreal Injections
- Platelet-Derived Growth Factor
(metabolism)
- Tacrolimus
(administration & dosage)
- Transforming Growth Factor beta
(metabolism)
- Vitreoretinopathy, Proliferative
(chemically induced, metabolism, prevention & control)
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