Abstract | BACKGROUND: METHODS: Twelve male New Zealand white rabbits were randomly divided into 2 groups. Using an alternative in situ lung I/R model, the left lung in the control group was supplied and preserved with LPDG solution for 120 minutes. In the study group 50,000 U/kg of ulinastatin was added to the LPDG solution for lung preservation. Then re-ventilation and reperfusion of the left lung were performed for 90 minutes. Blood gas analysis (PaO₂, PaCO₂), mean pulmonary artery pressure (MPAP) and serum TNF-α level were measured intermittently. The pulmonary water index (D/W), tissue myeloperoxidase (MPO) activity, tissue malondialdehyde (MDA) content and morphologic changes were analyzed. RESULTS: The study group showed significantly higher PaO₂ and lower MPAP at the end of reperfusion. Serum TNF-α level, left lung tissue MPO and MDA in the study group were significantly lower than those in the control group. D/W and pathologic evaluation were also remarkably different between the two groups. CONCLUSIONS: This study indicated that better lung preservation could be achieved with the use of an ulinastatin modified LPDG solution. Ulinastatin further attenuated lung I/R injury, at least partly by reducing oxidative reactions, inhibiting the release of inflammatory factors and neutrophils immigration.
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Authors | Ming Xu, Xiao-hong Wen, Shu-ping Chen, Xiao-xia An, He-yun Xu |
Journal | Chinese medical journal
(Chin Med J (Engl))
Vol. 124
Issue 14
Pg. 2179-83
(Jul 2011)
ISSN: 2542-5641 [Electronic] China |
PMID | 21933623
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Glycoproteins
- Organ Preservation Solutions
- urinastatin
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Topics |
- Animals
- Glycoproteins
(pharmacology)
- Lung
(drug effects, metabolism)
- Lung Transplantation
- Male
- Organ Preservation Solutions
(chemistry, pharmacology)
- Rabbits
- Random Allocation
- Reperfusion Injury
(prevention & control)
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