Abstract | OBJECTIVES: METHODS: Pancreatic MIAPaCa-2, Panc-1, cervical HeLa, and prostate cancer PC-3 cells were cultivated under normoxia or in hypoxic chamber. Expression of full-length TrpRS, antiangiogenic TrpRS, cyclin B1, hypoxia-inducible factor 1α, and Glut-1 was determined with reverse transcriptase-polymerase chain reaction, immunoblotting, and immunocytochemistry. RESULTS: We demonstrate that hypoxia regulates differentially TrpRS splice forms. Pronounced down-regulation of full-length TrpRS by hypoxia is concomitant with higher metastatic ability. CONCLUSIONS:
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Authors | Elena L Paley, Deana E Paley, Tatyana Merkulova-Rainon, Pochi R Subbarayan |
Journal | Pancreas
(Pancreas)
Vol. 40
Issue 7
Pg. 1043-56
(Oct 2011)
ISSN: 1536-4828 [Electronic] United States |
PMID | 21926542
(Publication Type: Journal Article)
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Chemical References |
- Biomarkers, Tumor
- CCNB1 protein, human
- Cyclin B1
- Glucose Transporter Type 1
- HIF1A protein, human
- Hypoxia-Inducible Factor 1, alpha Subunit
- Protein Isoforms
- RNA, Messenger
- SLC2A1 protein, human
- Tryptophan-tRNA Ligase
- WARS1 protein, human
- Oxygen
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Topics |
- Biomarkers, Tumor
(genetics, metabolism)
- Blotting, Western
- Cell Hypoxia
- Cyclin B1
(metabolism)
- Down-Regulation
- Gene Expression Regulation, Enzymologic
- Gene Expression Regulation, Neoplastic
- Glucose Transporter Type 1
(metabolism)
- HeLa Cells
- Humans
- Hypoxia-Inducible Factor 1, alpha Subunit
(metabolism)
- Immunohistochemistry
- Neoplasm Invasiveness
- Oxygen
(metabolism)
- Pancreatic Neoplasms
(enzymology, genetics, pathology)
- Protein Isoforms
- RNA, Messenger
(metabolism)
- Reverse Transcriptase Polymerase Chain Reaction
- Spheroids, Cellular
- Time Factors
- Tryptophan-tRNA Ligase
(genetics, metabolism)
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