Dietary induction of long-term hyperoxaluria in the porcine model.

Abstract

PURPOSE:

The purpose of our study was to determine if a hydroxyproline (HP) or gelatin diet could induce long-term hyperoxaluria in the porcine model.

MATERIALS AND METHODS:

A total of 18 gravid crossbred sows (Large White × Landrace) were randomly allotted into three treatment groups: 5% HP, 10% HP, and gelatin diet. All sows were catheterized 1 day before starting treatment diet. Catheters were left in place for 5 days before being removed. Sows were recatheterized for urine collections on days 11 to 12 and days 21 to 22. Urine was collected for each entire 24-hour period, and urinary oxalate was determined by ion chromatography.

RESULTS:

Urinary oxalate concentrations for all three diets peaked within the first 5 days of the diet. The sows fed the 5% HP, 10% HP, and gelatin diets had an early peak in urinary oxalate concentration (mg/L) at day 2 (158% increase), day 5 (316% increase), and day 5 (830% increase), respectively. The day 21 to 22 time points in all three diets demonstrated markedly increased urinary oxalate concentrations in comparison with baseline, with some concentrations higher than the early time point peaks (day 22: 5% HP=1906% increase, P=0.12; 10% HP=640% increase, P=0.02; gelatin=501% increase, P=0.01).

CONCLUSION:

Although both the 10% HP and gelatin diets induce significant short- and long-term hyperoxaluria in the porcine model, the gelatin diet is more cost-effective. The ability to induce long-term hyperoxaluria has important implications in establishing a porcine model for oxalate urolithiasis.

• Authors: Sutchin R Patel, Kristina L Penniston, Lauren Iwicki, Ibrahim Saeed, Thomas D Crenshaw, Stephen Y Nakada
• Journal: Journal of endourology (J Endourol) Vol. 26 Issue 5 Pg. 433-8 (May 2012) ISSN: 1557-900X [Electronic] United States
• PMID: 21923275 (Publication Type: Journal Article)

Chemical References

• Oxalates
• Gelatin
• Hydroxyproline

Topics

• Animals
• Catheterization
• Diet (adverse effects)
• Disease Models, Animal
• Gelatin (adverse effects)
• Hydroxyproline (adverse effects)
• Hyperoxaluria (chemically induced)
• Oxalates (urine)
• Sus scrofa
• Time Factors