Abstract | OBJECTIVES: METHODS: Rats were treated nasally with p1, p2 or phosphate-buffered saline before arthritis induction. Arthritis scores were assessed and peptide-specific proliferative responses, phenotypic analysis, cytokine production and in vitro suppressive capacity of cells were measured in lymph nodes and spleens. CD4 spleen T cells from p1- or p2-treated rats were adoptively transferred into naïve rats that were subsequently injected with complete Freund's adjuvant for arthritis induction. RESULTS: CONCLUSION: p1 immune therapy induces a population of CD4 T cells with reduced TNFα and increased peptide-specific IFNγ production at the site of inflammation. This population expresses FoxP3 and has potent suppressive capacity which, upon transfer, protects against arthritis. The bystander epitope p1 may therefore be a suitable candidate for antigen-specific immunotherapy in arthritis.
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Authors | Evelien Zonneveld-Huijssoon, Sarah T A Roord, Wilco de Jager, Mark Klein, Salvatore Albani, Stephen M Anderton, Wietse Kuis, Femke van Wijk, Berent J Prakken |
Journal | Annals of the rheumatic diseases
(Ann Rheum Dis)
Vol. 70
Issue 12
Pg. 2199-206
(Dec 2011)
ISSN: 1468-2060 [Electronic] England |
PMID | 21914624
(Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Chaperonin 60
- Cytokines
- Epitopes, T-Lymphocyte
- Inflammation Mediators
- Peptide Fragments
- Freund's Adjuvant
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Topics |
- Administration, Intranasal
- Animals
- Arthritis, Experimental
(immunology, prevention & control)
- Bystander Effect
(immunology)
- CD4-Positive T-Lymphocytes
(transplantation)
- Chaperonin 60
(administration & dosage, immunology, therapeutic use)
- Cytokines
(biosynthesis)
- Epitopes, T-Lymphocyte
(administration & dosage, immunology, therapeutic use)
- Freund's Adjuvant
- Immunity, Mucosal
- Immunotherapy, Adoptive
(methods)
- Inflammation Mediators
(metabolism)
- Lymphocyte Activation
(immunology)
- Male
- Peptide Fragments
(administration & dosage, immunology, therapeutic use)
- Rats
- Rats, Inbred Lew
- Spleen
(immunology)
- T-Lymphocytes
(immunology)
- T-Lymphocytes, Regulatory
(immunology)
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