Nanodiamond as a vector for siRNA delivery to Ewing sarcoma cells.

Abstract

The ability of diamond nanoparticles (nanodiamonds, NDs) to deliver small interfering RNA (siRNA) into Ewing sarcoma cells is investigated with a view to the possibility of in-vivo anticancer nucleic-acid drug delivery. siRNA is adsorbed onto NDs that are coated with cationic polymer. Cell uptake of NDs is demonstrated by taking advantage of the NDs' intrinsic fluorescence from embedded color-center defects. Cell toxicity of these coated NDs is shown to be low. Consistent with the internalization efficacy, a specific inhibition of EWS/Fli-1 gene expression is shown at the mRNA and protein level by the ND-vectorized siRNA in a serum-containing medium.

• Authors: Anna Alhaddad, Marie-Pierre Adam, Jacques Botsoa, Géraldine Dantelle, Sandrine Perruchas, Thierry Gacoin, Christelle Mansuy, Solange Lavielle, Claude Malvy, François Treussart, Jean-Rémi Bertrand
• Journal: Small (Weinheim an der Bergstrasse, Germany) (Small) Vol. 7 Issue 21 Pg. 3087-95 (Nov 04 2011) ISSN: 1613-6829 [Electronic] Germany
• PMID: 21913326 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Chemical References

• Nanodiamonds
• RNA, Small Interfering

Topics

• Animals
• Bone Neoplasms (genetics, therapy)
• Cell Line, Tumor
• Mice
• Microscopy, Fluorescence
• NIH 3T3 Cells
• Nanodiamonds
• RNA, Small Interfering (genetics)
• Sarcoma, Ewing (genetics, therapy)
• Transfection
• Xenograft Model Antitumor Assays