Comparative analysis of the immunohistochemical expression of collagen IV, MMP-9, and TIMP-2 in odontogenic cysts and tumors.

Abstract

OBJECTIVE:

The aim of this study was to evaluate the immunohistochemical expression of collagen IV, matrix metalloproteinase (MMP) 9 and tissue inhibitor of MMP (TIMP) 2 in dentigerous cysts (DCs), radicular cysts (RCs), keratocystic odontogenic tumors (KOTs), and ameloblastomas.

STUDY DESIGN:

Twenty cases of DCs, 20 RCs, 20 KOTs, and 20 ameloblastomas were selected and analyzed by immunohistochemistry.

RESULTS:

Most DCs and RCs showed continuous and >50% staining for collagen IV in the basement membrane of the epithelium, whereas predominantly discontinuous thin and ≤ 50% staining was observed in KOTs and ameloblastomas, with a significant difference in staining percentage (P < .001). MMP-9 was diffusely distributed and localized in both epithelial and mesenchymal cells of all of the lesions analyzed. The staining percentage was higher in the epithelium (P = .058) and mesenchyme (P = .005) of KOTs and ameloblastomas. Moreover, the distribution pattern, location, and percentage of expression of TIMP-2 were similar in the lesions studied, except for ameloblastoma, with a significant difference in staining percentage (P < .001).

CONCLUSION:

These results demonstrate that the interaction between collagen IV, MMP-9, and TIMP-2 is an important factor for the establishment of differences in the biologic behavior of the odontogenic cysts and tumors studied.

• Authors: Águida Cristina Gomes Henriques, Marcelo Gadelha Vasconcelos, Hébel Cavalcanti Galvão, Lélia Batista de Souza, Roseana de Almeida Freitas
• Journal: Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics (Oral Surg Oral Med Oral Pathol Oral Radiol Endod) Vol. 112 Issue 4 Pg. 468-75 (Oct 2011) ISSN: 1528-395X [Electronic] United States
• PMID: 21911204 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 Mosby, Inc. All rights reserved.

Chemical References

• Collagen Type IV
• Coloring Agents
• Protease Inhibitors
• TIMP2 protein, human
• Tissue Inhibitor of Metalloproteinase-2
• Matrix Metalloproteinase 9

Topics

• Ameloblastoma (enzymology, pathology)
• Basement Membrane (enzymology, pathology)
• Collagen Type IV (analysis)
• Coloring Agents
• Connective Tissue (enzymology, pathology)
• Dentigerous Cyst (enzymology, pathology)
• Endothelial Cells (enzymology, pathology)
• Epithelial Cells (enzymology, pathology)
• Epithelium (enzymology, pathology)
• Fibroblasts (enzymology, pathology)
• Humans
• Immunohistochemistry
• Matrix Metalloproteinase 9 (analysis)
• Mesoderm (enzymology, pathology)
• Odontogenic Cysts (enzymology, pathology)
• Protease Inhibitors (analysis)
• Radicular Cyst (enzymology, pathology)
• Tissue Inhibitor of Metalloproteinase-2 (analysis)