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Enhanced levels of both the membrane-bound and soluble forms of IgM Fc receptor (FcμR) in patients with chronic lymphocytic leukemia.

Abstract
The association of an IgM-Fc receptor (FcμR) with chronic lymphocytic leukemia (CLL) was suggested more than 30 years ago, but its authenticity has never been formally addressed. We examined the expression of the recently identified FcμR by B and T cells in CLL patients using receptor-specific monoclonal antibodies. CLL B cells (CD5(+)/CD19(+)) expressed much higher levels of FcμR on their cell surface than B cells from healthy donors. Such enhanced expression was more evident in immunoglobulin heavy chain variable region (IGHV)-mutated, CD38(-) or early Rai-stage CLL than in IGHV-unmutated, CD38(+), or advanced Rai-stage CLL. Intriguingly, surface FcμR levels also were significantly elevated in the non-CLL B cells (CD5(-)/CD19(+)) and T cells (CD5(+)/CD19(-)), especially in IGHV-mutated CLL. CLL patients also had high serum titers of FcμR compared with healthy donors, and serum FcμR levels correlated significantly with circulating lymphocyte numbers but not with the IGHV mutation status or Rai stage. The serum FcμR was resolved as an ∼ 40-kDa protein, distinct from the cell surface FcμR of ∼ 60 kDa, and it was produced by both CLL B and non-CLL B cells. Mass spectrometric analysis revealed that the serum FcμR is a soluble form of the receptor encoded by an alternatively spliced FcμR transcript. These findings indicate enhanced levels of both membrane-bound and soluble forms of FcμR in CLL patients.
AuthorsFu Jun Li, Yoshiki Kubagawa, Matthew K McCollum, Landon Wilson, Tomoko Motohashi, Luigi F Bertoli, James C Barton, Stephen Barnes, Randall S Davis, Hiromi Kubagawa
JournalBlood (Blood) Vol. 118 Issue 18 Pg. 4902-9 (Nov 03 2011) ISSN: 1528-0020 [Electronic] United States
PMID21908424 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
Chemical References
  • Antigens, Surface
  • Membrane Proteins
  • Protein Isoforms
  • Receptors, Fc
  • immunoglobulin M receptor
Topics
  • Amino Acid Sequence
  • Antigens, Surface (blood, genetics, metabolism)
  • Cells, Cultured
  • Gene Expression Regulation, Leukemic
  • Humans
  • Leukemia, Lymphocytic, Chronic, B-Cell (blood, genetics, metabolism)
  • Membrane Proteins (chemistry, genetics, metabolism)
  • Molecular Sequence Data
  • Protein Isoforms (blood, chemistry, genetics, metabolism)
  • Receptors, Fc (blood, chemistry, genetics, metabolism)
  • Solubility
  • Up-Regulation

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