Antimicrobial resistance of Staphylococcus spp. isolates from cases of mastitis in buffalo in Brazil.

Abstract

Persistent buffalo mastitis caused by Staphylococcus spp. gives rise to economic losses and may be resistant to antimicrobial therapy. The aim of the present study was to determine resistance patterns and the presence of mecA, blaZ, and efflux pump in Staphylococcus spp. isolated from cases of mastitis in Brazilian buffalo herds. Susceptibility to antimicrobials was determined by the disk diffusion test and detection of the mecA and blaZ genes by polymerase chain reaction. The efflux pump screening test was performed by growing samples in Muller-Hinton agar containing ethidium bromide. The percentages for resistance to the drugs tested were: 71.8% to penicillin, 49.2% to amoxicillin, 65.8% to oxacillin, 62.3% to cefquinome, 44.7% to cephalonium, 45.2% to ciprofloxacin, 32.6% to enrofloxacin, 58.7% to erythromycin, 42.7% to florfenicol, 34.6% to gentamicin, 35.1% to trimethoprim-sulfamethoxazole, 8.5% to tetracycline + neomycin + bacitracin, 43.2% to cephalothin, 38.1% to streptomycin, 58.7% to tetracycline, 31.6% to norfloxacin, 45.2% to ceftriaxone, 43.2% to nitrofurantoin, 57.7% to doxycycline, and 53.7% to cephalexin. Simultaneous resistance to 4 or more antimicrobial drug groups was observed in 112 isolates, using the mecA (11) and blaZ (79) genes, and efflux pump (47). It is concluded that Staphylococcus spp. isolates from cases of mastitis in Brazilian buffalo show varying levels of resistance to antibiotics, and caution should be exercised in choosing therapeutics in order to minimize the risk to public health.

• Authors: Elizabeth S de Medeiros, Chirles A França, Carina da C Krewer, Renata de M Peixoto, Aldo F de Souza Jr, Marielly B Cavalcante, Mateus M da Costa, Rinaldo A Mota
• Journal: Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc (J Vet Diagn Invest) Vol. 23 Issue 4 Pg. 793-6 (Jul 2011) ISSN: 1943-4936 [Electronic] United States
• PMID: 21908326 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Bacterial Agents

Topics

• Animals
• Anti-Bacterial Agents (pharmacology)
• Brazil (epidemiology)
• Buffaloes
• Drug Resistance, Bacterial
• Female
• Mastitis (epidemiology, microbiology, veterinary)
• Polymerase Chain Reaction (veterinary)
• Staphylococcal Infections (epidemiology, microbiology, veterinary)
• Staphylococcus (drug effects)