Interactome study suggests multiple cellular functions of hepatoma-derived growth factor (HDGF).

Abstract

Hepatoma-derived growth factor (HDGF) is a growth factor related to normal development and tumorigenesis; however, the mechanism of its mitogenic and angiogenic activity still remains unknown. Analysis of the HDGF interactome could be important for understanding its function and integrative mechanisms, because knowledge about HDGF interactors is very limited. In this study, through streptavidin-binding peptide (SBP) and Flag tag-based tandem affinity purification (SBP/Flag-TAP) coupled with LC-MS/MS, 106 proteins were shown to form complexes with HDGF. RNAs were also found in the HDGF complex through the SBP-tag based RNA co-immunoprecipitation (SBP-RIP) assay. Some of these interactions were confirmed by Co-IP and RT-PCR. We then found that the HATH domain was essential for HDGF interactions including protein-protein and protein-RNA interactions, and that in the absence of the HATH domain, NO-HATH could not form complex. The interactome suggests that HDGF is a multifunctional protein and participates in many cellular events, including ribosome biogenesis, RNA processing, DNA damage repair and transcriptional regulation. This new information about the HDGF interactome will further our understanding on HDGF-mediated cellular functions.

• Authors: Jian Zhao, Hongxiu Yu, Ling Lin, Jun Tu, Lili Cai, Yanmei Chen, Fan Zhong, Chengzhao Lin, Fuchu He, Pengyuan Yang
• Journal: Journal of proteomics (J Proteomics) Vol. 75 Issue 2 Pg. 588-602 (Dec 21 2011) ISSN: 1876-7737 [Electronic] Netherlands
• PMID: 21907836 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 Elsevier B.V. All rights reserved.

Chemical References

• Carrier Proteins
• Intercellular Signaling Peptides and Proteins
• hepatoma-derived growth factor
• streptavidin-binding peptide

Topics

• Carrier Proteins (metabolism)
• HEK293 Cells
• Humans
• Immunoprecipitation
• Intercellular Signaling Peptides and Proteins (genetics, physiology)
• Protein Interaction Domains and Motifs
• Tandem Mass Spectrometry
• Transcriptional Activation (drug effects, physiology)