[Application of melanocytes and bone marrow mesenchymal stem cells in tissue engineered skin construction].

Abstract

OBJECTIVE:

To explore the method of constructing tissue-engineered skin using melanocytes and bone marrow mesenchymal stem cells (BMSCs) in vivo.

METHODS:

Melanocytes were isolated from human foreskin. BMSCs were isolated from human bone marrow. Both of them were co-cultured at a ratio of 1:10, and then were implanted into the collagen membrane to construct the tissue-engineered skin, which was applied for wound repair in nude mice. The effectiveness of wound repair and the distribution of melanocytes were evaluated by morphological observation, in vivo 4,6-diamidino-2-phenylindole, dihydrochloride (DAPI) fluorescent staining tracing, HE staining, S-100 immunohistochemistry, and transmission electron microscopy.

RESULTS:

The wounds were satisfactorily repaired among the nude mice. The melanocytes were distributed in the skin with normal structure, as confirmed by DAPI fluorescent staining tracing, HE staining, S-100 immunohistochemistry, and transmission electron microscopy.

CONCLUSION:

Melanocytes and BMSCs, after proper in vitro culture at an appropriate ratio, can construct the tissue-engineered skin with I type collagen membrane.

• Authors: Ke-xin Song, Qun Qiao, Da-qing Liu, Xiao-jun Wang, Ru Zhao, Zhi-fei Liu, Xue-tao Pei
• Journal: Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae (Zhongguo Yi Xue Ke Xue Yuan Xue Bao) Vol. 33 Issue 4 Pg. 402-7 (Aug 2011) ISSN: 1000-503X [Print] China
• PMID: 21906449 (Publication Type: Journal Article)

Chemical References

• Collagen Type I

Topics

• Animals
• Bone Marrow Cells (cytology)
• Cells, Cultured
• Coculture Techniques
• Collagen Type I
• Humans
• Melanocytes (cytology)
• Mesenchymal Stem Cells (cytology)
• Mice
• Mice, Nude
• Skin (injuries)
• Skin, Artificial
• Tissue Engineering