[Effect of phospholipomannan of Candida albicans on the production of interleukin 6 and interleukin 8 in monocytes].

Abstract

OBJECTIVE:

To investigate whether Candida albicans-native phospholipomannan (PLM) induce an inflammation response through Toll-like receptor(TLRé2 in human acute monocytic leukemia cell line (THP-1) cells.

METHODS:

Human THP-1 monocytes were challenged with PLM in vitro. The mRNA expressions of TLR2, TLR4, proinflammatory cytokine [interleukin(IL)-6], and chemokine (IL-8) were assayed by real time reverse transcription polymerase chain reaction. The secretions of IL-6 and IL-8 were measured by enzyme-linked immunosorbent assay. The expression of TLR2 was analyzed with Western blot.

RESULTS:

PLM increased the mRNA expressions and secretions of proinflammatory cytokines (IL-6) and chemokines (IL-8) in THP-1 cells (all P=0.0000). PLM up-regulated the mRNA and protein levels of TLR2 (P=0.0000), whereas the mRNA level of TLR4 was not altered. PLM hydrolyzed with β-D-mannoside manno hydrolase failed to induce gene and protein expressions of TLR2, IL-6, and IL-8. Anti-TLRS-neutralizing antibody blocked the PLM-induced secretions of IL-6 and IL-8 in THP-1 cells (P = 0.0003, P = 0.0010).

CONCLUSION:

Canidada albicans-native PLM may contribute to the inflammatory responses during Candida infection in a TLR2-dependent manner.

• Authors: Qing Chen, Min Li, Rong-cai Tang, Wei-da Liu, Wu-qing Zhou, Yong-nian Shen, Gui-xia Lv
• Journal: Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae (Zhongguo Yi Xue Ke Xue Yuan Xue Bao) Vol. 33 Issue 4 Pg. 371-4 (Aug 2011) ISSN: 1000-503X [Print] China
• PMID: 21906443 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• CXCL8 protein, human
• Glycolipids
• IL6 protein, human
• Interleukin-6
• Interleukin-8
• TLR2 protein, human
• TLR4 protein, human
• Toll-Like Receptor 2
• Toll-Like Receptor 4
• phospholipomannan

Topics

• Candida albicans (chemistry)
• Cells, Cultured
• Glycolipids (pharmacology)
• Humans
• Interleukin-6 (metabolism)
• Interleukin-8 (metabolism)
• Monocytes (drug effects, immunology, metabolism)
• Toll-Like Receptor 2 (metabolism)
• Toll-Like Receptor 4 (metabolism)