Catechins have a great variety of
biological actions. We evaluated the potential benefits of
catechin ingestion on muscle contractile properties, oxidative stress, and
inflammation following downhill running, which is a typical eccentric exercise, in senescence-accelerated prone mice (
SAMP). Downhill running (13 m/min for 60 min; 16° decline) induced a greater decrease in the contractile force of soleus muscle and in Ca(2+)-
ATPase activity in SAMP1 compared with the senescence-resistant mice (SAMR1). Moreover, compared with SAMR1, SAMP1 showed greater downhill running-induced increases in plasma CPK and LDH activity,
malondialdehyde, and carbonylated
protein as markers of oxidative stress; and in
protein and
mRNA expression levels of the inflammatory mediators such as
tumor necrosis factor-α and
monocyte chemoattractant protein-1 in muscle. SAMP1 exhibited aging-associated vulnerability to oxidative stress and
inflammation in muscle induced by downhill running. Long-term (8 wk)
catechin ingestion significantly attenuated the downhill running-induced decrease in muscle force and the increased inflammatory mediators in both plasma and gastrocnemius muscle. Furthermore, catechins significantly inhibited the increase in oxidative stress markers immediately after downhill running, accompanied by an increase in
glutathione reductase activity. These findings suggest that long-term
catechin ingestion attenuates the aging-associated loss of force production, oxidative stress, and
inflammation in muscle after exercise.