Antibacterial effects of chemomechanical instrumentation and calcium hydroxide in primary teeth with pulp necrosis.

Abstract

PURPOSE:

This study's purposes were to: evaluate the antibacterial effect of chemomechanical instrumentation and a calcium hydroxide-based dressing in primary teeth with pulp necrosis secondary to trauma; and detect the presence of Fusobacterium nucleatum and black-pigmented rods in the canals of these teeth.

METHODS:

Microbiological sample collections (MSCs) were obtained: after coronal access to the canals of primary incisors (in 18 teeth; MSC 1); after chemomechanical instrumentation (in 10 teeth; MSC 2); and 72 hours after removal of intracanal medication (in 18 teeth; MSC 3). These samples were sent for microbiological processing. The results were analyzed statistically via chi-square, analysis of variance, and Games-Howell tests (P<.05).

RESULTS:

Micro-organisms were isolated in approximately 94%, 10%, and 83% of canals, respectively, in MSCs 1, 2, and 3. There was a statistically significant difference only between MSCs 1 and 2 (P<.03). F nucleatum and black-pigmented rods were detected in approximately 56% and 11% of canals in MSC 1, respectively.

CONCLUSION:

Chemomechanical instrumentation and calcium hydroxide-based dressing have an anti-bacterial effect by significantly reducing the number of micro-organisms in the main root canal. They showed a limited efficacy, however, and did not prevent bacterial regrowth after endodontic therapy in primary teeth with pulp necrosis secondary to trauma.

• Authors: Denise Lins de Sousa, Rebecca Bastos Rocha Araújo de Sousa, Daniela Nunes Pinto, José Jeová Siebra Moreira Neto, Cibele Barreto Mano de Carvalho, Paulo César de Almeida
• Journal: Pediatric dentistry (Pediatr Dent) 2011 Jul-Aug Vol. 33 Issue 4 Pg. 307-11 ISSN: 0164-1263 [Print] United States
• PMID: 21902996 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Infective Agents, Local
• Root Canal Irrigants
• Sodium Hypochlorite
• Calcium Hydroxide
• Chlorhexidine

Topics

• Analysis of Variance
• Anti-Infective Agents, Local (pharmacology)
• Calcium Hydroxide (pharmacology)
• Child
• Child, Preschool
• Chlorhexidine (pharmacology)
• Cross-Sectional Studies
• Dental Pulp Necrosis (etiology, microbiology, therapy)
• Female
• Fusobacterium nucleatum (drug effects)
• Gram-Negative Anaerobic Straight, Curved, and Helical Rods (drug effects)
• Humans
• Male
• Root Canal Irrigants (pharmacology)
• Root Canal Preparation (instrumentation, methods)
• Sodium Hypochlorite (pharmacology)
• Statistics, Nonparametric
• Tooth Injuries (complications)
• Tooth, Deciduous