Disruption of microenvironment and decrease in
oxygen supply during isolation and culture lead to pancreatic islet injury and their poor survival after
transplantation. This study aimed to create a matrix for culturing islets, using
fibrin as scaffold and
perfluorodecalin as
oxygen diffusion enhancing medium. Human pancreatic islets were divided in four groups: control, islets cultured in
fibrin, islets in
fibrin containing non-emulsified
perfluorodecalin, and finally islets in
fibrin supplemented with emulsified
perfluorodecalin. After an overnight culture, cell damage (viability,
proinsulin and
insulin unregulated release, apoptosis (
caspase-3 activation), secretory function, and presence of
hypoxia markers (HIF-1a and
VEGF expression) were assessed. Islets cultured in a matrix, had similar islet viability to controls (no matrix) but decreased levels of active
caspase-3 and unregulated
hormone release, but high level of
hypoxia markers expression. Although the supplementation of
fibrin with non-emulsified
perfluorodecalin improves secretory response, there was no decrease in
hypoxia markers expression. In contrast, emulsified
perfluorodecalin added to the matrix improved islet function, islet viability and maintained level of
hypoxia markers similar to control.
Fibrin matrix supplemented with emulsified
perfluorodecalin can provide a beneficial physical and chemical environment for improved pancreatic human islet function and viability in vitro.