Abstract | BACKGROUND AND OBJECTIVE: Early detection of colon adenomas at high risk of progression and early-stage colorectal cancer (CRC) is an effective approach to reduce CRC death rates. Current screening methods lack specificity as they detect many adenomas that will never progress to CRC. The authors aimed to identify cell surface protein biomarkers with extracellular domains that could be targeted for molecular imaging and discriminate low-risk adenomas and normal colon from high-risk adenomas and CRC. DESIGN:
Cell surface proteins of five CRC cell lines were biotinylated, isolated and analysed by in-depth proteomics using gel electrophoresis and nanoliquid chromatography coupled to tandem mass spectrometry. Differential expression in adenomas and CRCs was based on mRNA expression and verified by immunohistochemical staining of tissue microarrays. RESULTS: CONCLUSION:
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Authors | Meike de Wit, Connie R Jimenez, Beatriz Carvalho, Jeroen A M Belien, Pien M Delis-van Diemen, Sandra Mongera, Sander R Piersma, Maindad Vikas, Sanjay Navani, Fredrik Pontén, Gerrit A Meijer, Remond J A Fijneman |
Journal | Gut
(Gut)
Vol. 61
Issue 6
Pg. 855-64
(Jun 2012)
ISSN: 1468-3288 [Electronic] England |
PMID | 21890811
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biomarkers
- Glucose Transporter Type 1
- Neoplasm Proteins
- PrPC Proteins
- Prions
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Topics |
- Adenoma
(chemistry, diagnosis)
- Biomarkers
(analysis)
- Blotting, Western
- Caco-2 Cells
(chemistry)
- Carcinoma
(chemistry, diagnosis)
- Cell Line, Tumor
- Colorectal Neoplasms
(chemistry, diagnosis)
- DNA Copy Number Variations
- Disease Progression
- Electrophoresis, Polyacrylamide Gel
- Glucose Transporter Type 1
(analysis)
- HT29 Cells
(chemistry)
- Humans
- Neoplasm Proteins
(analysis)
- PrPC Proteins
(analysis)
- Prions
(analysis)
- Protein Array Analysis
(methods)
- Proteomics
(methods)
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