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Amidated joining peptide in the human pituitary, gut, adrenal gland and bronchial carcinoids. Immunocytochemical and immunochemical evidence.

Abstract
The distribution of the proopiomelanocortin-derivated amidated joining peptide (JP-N) was examined in the human pituitary gland, adrenal gland, gut and in three bronchial carcinoids. Double immunostaining showed coexistence of immunoreactive JP-N and other proopiomelanocortin derivatives, e.g., ACTH, beta-endorphin, Pro-tau-MSH, in the pituitary gland and adrenal medulla. The JP-N immunoreactive cells in the adrenal medulla were identified as a subpopulation of adrenaline-producing cells by means of an antiserum against phenylethanolamine N-methyltransferase. In the gut immunoreactive JP-N was costored with somatostatin in endocrine cells. Using radioimmunoassay, JP-N was found in higher concentrations than ACTH and alpha-MSH in the gut but not in the adrenal gland. Gel chromatography of gastric antrum and adrenal gland extracts showed three and two dominating components of immunoreactive JP-N, respectively, but under reduced conditions most of the immunoreactive material appeared as of low molecular weight in both extracts. In conclusion, immunoreactive JP-N is a major product from the processing of proopiomelanocortin in human extrapituitary tissues. The molecular forms of immunoreactive JP-N correspond to previous findings in the human pituitary gland.
AuthorsA Bjartell, M Fenger, R Ekman, F Sundler
JournalPeptides (Peptides) 1990 Jan-Feb Vol. 11 Issue 1 Pg. 149-61 ISSN: 0196-9781 [Print] United States
PMID2188229 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Peptide Fragments
  • Pro-Opiomelanocortin
  • pro-opiomelanocortin joining peptide(79-108)
Topics
  • Adrenal Glands (metabolism)
  • Amino Acid Sequence
  • Bronchial Neoplasms (analysis)
  • Carcinoid Tumor (analysis)
  • Chromatography, Gel
  • Digestive System (analysis)
  • Fluorescent Antibody Technique
  • Humans
  • Immunochemistry
  • Immunoenzyme Techniques
  • Molecular Sequence Data
  • Peptide Fragments
  • Pituitary Gland (analysis)
  • Pro-Opiomelanocortin
  • Radioimmunoassay

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