Abstract |
The distribution of the proopiomelanocortin-derivated amidated joining peptide (JP-N) was examined in the human pituitary gland, adrenal gland, gut and in three bronchial carcinoids. Double immunostaining showed coexistence of immunoreactive JP-N and other proopiomelanocortin derivatives, e.g., ACTH, beta-endorphin, Pro-tau- MSH, in the pituitary gland and adrenal medulla. The JP-N immunoreactive cells in the adrenal medulla were identified as a subpopulation of adrenaline-producing cells by means of an antiserum against phenylethanolamine N-methyltransferase. In the gut immunoreactive JP-N was costored with somatostatin in endocrine cells. Using radioimmunoassay, JP-N was found in higher concentrations than ACTH and alpha-MSH in the gut but not in the adrenal gland. Gel chromatography of gastric antrum and adrenal gland extracts showed three and two dominating components of immunoreactive JP-N, respectively, but under reduced conditions most of the immunoreactive material appeared as of low molecular weight in both extracts. In conclusion, immunoreactive JP-N is a major product from the processing of proopiomelanocortin in human extrapituitary tissues. The molecular forms of immunoreactive JP-N correspond to previous findings in the human pituitary gland.
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Authors | A Bjartell, M Fenger, R Ekman, F Sundler |
Journal | Peptides
(Peptides)
1990 Jan-Feb
Vol. 11
Issue 1
Pg. 149-61
ISSN: 0196-9781 [Print] United States |
PMID | 2188229
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Peptide Fragments
- Pro-Opiomelanocortin
- pro-opiomelanocortin joining peptide(79-108)
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Topics |
- Adrenal Glands
(metabolism)
- Amino Acid Sequence
- Bronchial Neoplasms
(analysis)
- Carcinoid Tumor
(analysis)
- Chromatography, Gel
- Digestive System
(analysis)
- Fluorescent Antibody Technique
- Humans
- Immunochemistry
- Immunoenzyme Techniques
- Molecular Sequence Data
- Peptide Fragments
- Pituitary Gland
(analysis)
- Pro-Opiomelanocortin
- Radioimmunoassay
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