Abstract | ETHNOPHARMACOLOGICAL RELEVANCE: AIM OF THE STUDY: In this study, we investigated the synergistic anti-inflammatory effect of the combination of SF and OMT, and its modulation on inflammation-associated mediators in RAW 264.7 cells. MATERIALS AND METHODS: RESULTS: The combination of SF and OMT could significantly inhibit the edema in the xylene-induced mouse ear edema and carrageenan-induced rat paw edema, but no effect was found when each drug was used alone according to above doses. The combination exhibited a better effect in down-regulating mRNA expressions of inflammation-associated mediators in LPS-stimulated RAW 264.7 cells than SF or OMT alone. The ELISA results showed that the combination synergistically inhibited LPS-induced IL-11, CRP and INF-γ production in a dose-dependent manner. CONCLUSION: The combination of SF and OMT showed synergistic anti-inflammatory effect, and the activity was probably related to its modulation on inflammation-associated mediators, especially IL-11, CRP and INF-γ.
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Authors | Xiaopei Yuan, Yan Sun, Ningshu Miao, Shuyu Sun, Yuyun Wang, Zhao Hu, Jing Yuan, Mingbo Xu, Zhifeng Liu |
Journal | Journal of ethnopharmacology
(J Ethnopharmacol)
Vol. 137
Issue 3
Pg. 1477-85
(Oct 11 2011)
ISSN: 1872-7573 [Electronic] Ireland |
PMID | 21878383
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2011 Elsevier Ireland Ltd. All rights reserved. |
Chemical References |
- Alkaloids
- Anti-Inflammatory Agents
- Coumaric Acids
- Cytokines
- Inflammation Mediators
- Interleukin-11
- Lipopolysaccharides
- Quinolizines
- RNA, Messenger
- Xylenes
- Interferon-gamma
- oxymatrine
- Carrageenan
- C-Reactive Protein
- ferulic acid
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Topics |
- Alkaloids
(pharmacology)
- Angelica sinensis
- Animals
- Anti-Inflammatory Agents
(pharmacology)
- C-Reactive Protein
(metabolism)
- Carrageenan
- Coumaric Acids
(pharmacology)
- Cytokines
(genetics, metabolism)
- Disease Models, Animal
- Dose-Response Relationship, Drug
- Down-Regulation
- Drug Synergism
- Edema
(chemically induced, immunology, prevention & control)
- Enzyme-Linked Immunosorbent Assay
- Female
- Inflammation Mediators
(metabolism)
- Interferon-gamma
(metabolism)
- Interleukin-11
(metabolism)
- Lipopolysaccharides
(pharmacology)
- Macrophages
(drug effects, immunology)
- Male
- Mice
- Plants, Medicinal
- Quinolizines
(pharmacology)
- RNA, Messenger
(metabolism)
- Rats
- Rats, Sprague-Dawley
- Real-Time Polymerase Chain Reaction
- Sophora
- Xylenes
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