Abstract |
Crustacean cardioactive peptide (CCAP), a cyclic nonapeptide (PFCNAFTGCamide), has multifunctional roles in insects including stimulating visceral and cardiac muscle contraction, and regulating ecdysis. Previously, we have sequenced the cDNA for CCAP from Rhodnius prolixus central nervous system (CNS) and shown expression of the CCAP transcript in neurons of the CNS. In the present study, we have biochemically identified and sequenced CCAP from 5th instar R. prolixus CNS using matrix-assisted laser desorption ionization-time of flight-tandem mass spectrometry, and mapped CCAP-like immunoreactivity in the CNS and peripheral tissues of 5th instar R. prolixus. Physiologically, the hindgut of R. prolixus was found to be sensitive to CCAP, showing dose-dependent increases in contractions with threshold at 5 × 10(-9) M and maximum response at 10(-7) M CCAP. Also, CCAP was found to increase the frequency of the heartbeat in a reversible, dose-dependent manner, with threshold close to 10(-11) M and maximum response at 10(-10) M CCAP.
|
Authors | Do Hee Lee, Angela B Lange |
Journal | General and comparative endocrinology
(Gen Comp Endocrinol)
Vol. 174
Issue 1
Pg. 36-43
(Oct 01 2011)
ISSN: 1095-6840 [Electronic] United States |
PMID | 21875591
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Copyright | Copyright © 2011 Elsevier Inc. All rights reserved. |
Chemical References |
- Neuropeptides
- crustacean cardioactive peptide
|
Topics |
- Animals
- Central Nervous System
(metabolism)
- Chagas Disease
(transmission)
- Disease Vectors
- Gastrointestinal Tract
(drug effects, metabolism)
- Heart
(drug effects)
- Immunohistochemistry
- Neurons
(metabolism)
- Neuropeptides
(metabolism, pharmacology)
- Rhodnius
(metabolism)
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
|