Abstract | BACKGROUND/AIM: MATERIALS AND METHODS: Samples (500 μl) from leukemic cells were loaded onto an anion-exchange column and eluted with a phosphate- acetonitrile buffer (flow rate: 0.7 ml/min) at ambient temperature. The Cl- F-ara-ATP concentration was determined by measuring absorbance at 254 nm. RESULTS: The standard curve was linear, with minimal within-day and inter-day variability. Recovery was excellent; low and high quantitation limits were 10 pmol and 5,000 pmol, respectively. Cl- F-ara-ATP eluted independently of ATP, GTP, UTP, and CTP. Production of Cl- F-ara-ATP was successfully measured in cultured leukemia HL-60 cells treated in vitro with Cl-F-ara-A. CONCLUSION: This method is simple, sensitive and applicable for determination of the Cl- F-ara-ATP content of biological materials.
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Authors | Takahiro Yamauchi, Rie Nishi, Takanori Ueda |
Journal | Anticancer research
(Anticancer Res)
Vol. 31
Issue 9
Pg. 2863-7
(Sep 2011)
ISSN: 1791-7530 [Electronic] Greece |
PMID | 21868530
(Publication Type: Journal Article, Validation Study)
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Chemical References |
- Adenine Nucleotides
- Antineoplastic Agents
- Arabinonucleosides
- Clofarabine
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Topics |
- Adenine Nucleotides
(analysis)
- Antineoplastic Agents
(analysis)
- Arabinonucleosides
(analysis)
- Chromatography, High Pressure Liquid
(methods)
- Clofarabine
- HL-60 Cells
- Humans
- Leukemia
(pathology)
- Reproducibility of Results
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