Inactivation of TLR9 by a suppressive oligodeoxynucleotides can ameliorate the clinical signs of EAN.

Abstract

Susceptible-strain animals immunized with P2 peptide could generate the disease of experimental autoimmune neuritis (EAN) with inflammation and demyelination of peripheral nerve. A myriad of transcription factors and inflammatory cytokines have been found to participate in this process; however, the roles of toll-like receptors (TLRs) are poorly understood in EAN. The aim of this study is to explore the role of TLR9 in the pathogenesis of EAN. The EAN was induced in Lewis rat by immunization with P2(53-78) and complete Freund's adjuvant. CpG oligodeoxynucleotides (ODN) (cODN), a suppressive ODN (sODN) and a control non-specific ODN (nODN) were respectively administered to explore the role of TLR9 in EAN both in vivo and vitro. Following immunization up to the peak phase of EAN, EAN rats inoculated with sODN had remarkably better clinical score of EAN and expressed a significantly inhibited TLR9 signaling pathway. Our study suggests that TLR9 may be involved in the pathogenesis of EAN.

• Authors: Yu-Zhong Wang, Qiu-Hua Liang, Hhoonisha Ramkalawan, Wei Zhang, Wen-Bin Zhou, Bo Xiao, Fa-Fa Tian, Huan Yang, Jing Li, Yong Zhang, Ning-An Xu
• Journal: Immunological investigations (Immunol Invest) Vol. 41 Issue 2 Pg. 171-82 ( 2012) ISSN: 1532-4311 [Electronic] England
• PMID: 21864114 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Myelin P2 Protein
• Oligodeoxyribonucleotides, Antisense
• Peptide Fragments
• Toll-Like Receptor 9
• myelin P2 protein (53-78)
• Freund's Adjuvant

Topics

• Animals
• Disease Models, Animal
• Disease Progression
• Disease Susceptibility
• Freund's Adjuvant (administration & dosage)
• Guillain-Barre Syndrome (immunology, therapy)
• Humans
• Immunization
• Male
• Myelin P2 Protein (administration & dosage)
• Neuritis, Autoimmune, Experimental (chemically induced, genetics, immunology, physiopathology, therapy)
• Oligodeoxyribonucleotides, Antisense (administration & dosage, adverse effects)
• Peptide Fragments (administration & dosage)
• Rats
• Rats, Inbred Lew
• Toll-Like Receptor 9 (genetics, metabolism)