Abstract | PURPOSE: DESIGN: Prospective, controlled, longitudinal study with 1-year follow-up. PARTICIPANTS: METHODS: Subjects received 4 consecutive monthly unilateral IVT injections and then were treated as needed. Each subject was assigned randomly to 1 of 4 antibiotics ( azithromycin 1%, gatifloxacin 0.3%, moxifloxacin 0.5%, ofloxacin 0.3%) and used only their assigned antibiotic after each injection. Conjunctival culture specimens of the treated and untreated fellow eye (control) were obtained at baseline and after each injection. All bacterial isolates were tested for antibiotic susceptibility to 16 different antibiotics using the Kirby-Bauer disc diffusion technique. MAIN OUTCOME MEASURES:
Antibiotic susceptibility patterns and multidrug resistance of S. epidermidis. RESULTS: After 4 consecutive treatments, a total of 58 isolates of S. epidermidis each were isolated from control and treated eyes. Resistance to 3 or more antibiotics was present in 69% of S. epidermidis isolated from control eyes compared with 90% from treated eyes (P<0.02). A total of 46 and 38 isolates of S. epidermidis were cultured from control and treated eyes, respectively, from the fifth until the final injection. Resistance to 5 or more antibiotics was present in 48% of control eyes compared with 71% of treated eyes (P<0.05). In a significant number of fluoroquinolone-treated eyes, S. epidermidis developed resistance to third-generation (P<0.01) and fourth-generation (P<0.01) fluoroquinolones compared with control eyes. In addition, these organisms developed resistance to trimethoprim/sulfamethoxazole (P<0.01), gentamicin (P<0.03), and clindamycin (P<0.05). Similarly, a significant number of azithromycin-treated eyes developed S. epidermidis resistant to macrolides (P<0.01) compared with control eyes and also developed increased resistance to trimethoprim/sulfamethoxazole (P<0.02) and doxycycline (P<0.01). CONCLUSIONS: FINANCIAL DISCLOSURE(S): Proprietary or commercial disclosure may be found after the references.
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Authors | Sarita B Dave, Hassanain S Toma, Stephen J Kim |
Journal | Ophthalmology
(Ophthalmology)
Vol. 118
Issue 10
Pg. 2035-40
(Oct 2011)
ISSN: 1549-4713 [Electronic] United States |
PMID | 21856006
(Publication Type: Comparative Study, Journal Article, Randomized Controlled Trial, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2011 American Academy of Ophthalmology. Published by Elsevier Inc. All rights reserved. |
Chemical References |
- Anti-Bacterial Agents
- Aza Compounds
- Fluoroquinolones
- Quinolines
- Azithromycin
- Ofloxacin
- Gatifloxacin
- Moxifloxacin
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Topics |
- Adult
- Anti-Bacterial Agents
(administration & dosage, therapeutic use)
- Aza Compounds
(administration & dosage, therapeutic use)
- Azithromycin
(administration & dosage, therapeutic use)
- Choroidal Neovascularization
(drug therapy)
- Conjunctiva
(microbiology)
- Drug Resistance, Multiple, Bacterial
- Fluoroquinolones
(administration & dosage, therapeutic use)
- Follow-Up Studies
- Gatifloxacin
- Humans
- Intravitreal Injections
- Microbial Sensitivity Tests
- Moxifloxacin
- Ofloxacin
(administration & dosage, therapeutic use)
- Prospective Studies
- Quinolines
(administration & dosage, therapeutic use)
- Staphylococcus epidermidis
(drug effects, isolation & purification)
- Young Adult
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