Connective tissue growth factor, a regulator related with 10-hydroxy-2-decenoic acid down-regulate MMPs in rheumatoid arthritis.

Abstract

10-hydroxy-2-decenoic acid (10H2DA) is suggested to be a potential medication for rheumatoid arthritis (RA) by activation of matrix metalloproteinases (MMPs) via mitogen-activated protein kinase signaling pathways. The aim of the present work was to seek differentially expressed proteins in rheumatoid arthritis synovial fibroblasts (RASFs) treated with 10H2DA by comparative proteomics analysis. Two-dimensional electrophoresis (2-DE) and LC-MS/MS were performed to identify changes in protein expression after 24-h 10H2DA treatment. Differentially expressed proteins were identified by real-time PCR and Western blot analysis. Influence of down-regulation of connective tissue growth factor (CTGF) expression on MMPs was studied by RNAi. Ten proteins were up-regulated and 9 proteins were down-regulated after 24-h 10H2DA treatment. A total of 19 differentially expressed proteins were identified and found to be associated with glycolysis, lipid metabolism, cell adhesion, ATP synthesis, oxidation reduction, and anti-apoptosis. CTGF, a member of the C-terminal cystein-rich proteins (CCN) family, was down-regulated after 24-h 10H2DA treatment. MMPs were down-regulated after RNAi (CTGFi). These results suggest that CTGF is a regulator factor in the expression of MMPs, and 10H2DA down-regulate the concentration of MMPs probably by down-regulating the expression of CTGF.

• Authors: Jian-guang Wang, Jian Ruan, Chun-yang Li, Jian-min Wang, Yan Li, Wei-tao Zhai, Wei Zhang, Hui Ye, Nian-han Shen, Kang-fu Lei, Xiu-fang Chen, Xin-yu Yang
• Journal: Rheumatology international (Rheumatol Int) Vol. 32 Issue 9 Pg. 2791-9 (Sep 2012) ISSN: 1437-160X [Electronic] Germany
• PMID: 21850473 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Fatty Acids, Monounsaturated
• RNA, Small Interfering
• Connective Tissue Growth Factor
• 10-hydroxy-2-decenoic acid
• Matrix Metalloproteinases

Topics

• Arthritis, Rheumatoid (metabolism, pathology)
• Cell Proliferation (drug effects)
• Cell Survival (drug effects)
• Cells, Cultured
• Connective Tissue Growth Factor (drug effects, genetics, metabolism)
• Down-Regulation (drug effects)
• Fatty Acids, Monounsaturated (pharmacology)
• Humans
• Matrix Metalloproteinases (drug effects, metabolism)
• Proteomics
• RNA, Small Interfering (pharmacology)
• Synovial Membrane (drug effects, metabolism, pathology)