A broadly neutralizing human monoclonal antibody that recognizes a conserved, novel epitope on the globular head of the influenza H1N1 virus hemagglutinin.

Abstract

The conserved influenza virus hemagglutinin (HA) stem domain elicits cross-reactive antibodies, but epitopes in the globular head typically elicit strain-specific responses because of the hypervariability of this region. We isolated human monoclonal antibody 5J8, which neutralized a broad spectrum of 20th century H1N1 viruses and the 2009 pandemic H1N1 virus. Fine mapping of the interaction unexpectedly revealed a novel epitope between the receptor-binding pocket and the Ca₂ antigenic site on HA. This antibody exposes a new mechanism underlying broad immunity against H1N1 influenza viruses and identifies a conserved epitope that might be incorporated into engineered H1 virus vaccines.

• Authors: Jens C Krause, Tshidi Tsibane, Terrence M Tumpey, Chelsey J Huffman, Christopher F Basler, James E Crowe Jr
• Journal: Journal of virology (J Virol) Vol. 85 Issue 20 Pg. 10905-8 (Oct 2011) ISSN: 1098-5514 [Electronic] United States
• PMID: 21849447 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Antibodies, Monoclonal
• Antibodies, Neutralizing
• Antibodies, Viral
• Epitopes
• H1N1 virus hemagglutinin
• Hemagglutinin Glycoproteins, Influenza Virus

Topics

• Adult
• Animals
• Antibodies, Monoclonal (immunology, therapeutic use)
• Antibodies, Neutralizing (immunology, therapeutic use)
• Antibodies, Viral (immunology, therapeutic use)
• Body Weight
• Cell Line
• Cross Reactions
• Disease Models, Animal
• Epitope Mapping
• Epitopes (immunology)
• Female
• Hemagglutinin Glycoproteins, Influenza Virus (immunology)
• Humans
• Mice
• Mice, Inbred BALB C
• Molecular Sequence Data
• Orthomyxoviridae Infections (drug therapy)
• Rodent Diseases (drug therapy)
• Sequence Analysis, DNA
• Survival Analysis
• Treatment Outcome