Cloning and expression analysis of a long type peptidoglycan recognition protein (PGRP-L) from Xenopus tropicalis.

Abstract

Peptidoglycan recognition proteins (PGRPs) are a family of pattern recognition receptors (PRRs) of the immune system, which bind and hydrolyze bacterial peptidoglycan. Here, a long type PGRP (PGRP-L) was first cloned in the lower vertebrate species Xenopus tropicalis (Xt). The XtPGRP-L possessed a conserved genomic structure with five exons and four introns. The alignment and phylogenetic analysis indicated that XtPGRP-L might be a type of amidase-like PGRP. The 3-D model showed that XtPGRP-L possessed a conserved structure compared with the Drosophila PGRP-Lb. During embryonic development, XtPGRP-L was not expressed until the 72 h tadpole stage. In adult tissues, it was strongly expressed in the liver, lung, intestine, and stomach. Furthermore, after LPS stimulation, the expression of XtPGRP-L was up-regulated significantly in the liver, intestine and spleen, indicating that XtPGRP-L may play an important role in the innate immunity of Xenopus tropicalis.

• Authors: Zhi-Tao Qi, Qi-Huan Zhang, Zi-Sheng Wang, Ai-Min Wang, Bei Huang, Ming-Xian Chang, Pin Nie
• Journal: Dong wu xue yan jiu = Zoological research (Dongwuxue Yanjiu) Vol. 32 Issue 4 Pg. 371-8 (Aug 2011) ISSN: 0254-5853 [Print] China
• PMID: 21842532 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Carrier Proteins
• Drosophila Proteins
• peptidoglycan recognition protein

Topics

• Amino Acid Sequence
• Animals
• Carrier Proteins (chemistry, genetics, metabolism)
• Cloning, Molecular
• Drosophila (chemistry, genetics)
• Drosophila Proteins (chemistry, genetics)
• Gene Expression Regulation
• Humans
• Molecular Sequence Data
• Phylogeny
• Sequence Homology, Amino Acid
• Xenopus (classification, genetics, metabolism)