Porphyromonas gingivalis-mediated shedding of extracellular matrix metalloproteinase inducer (EMMPRIN) by oral epithelial cells: a potential role in inflammatory periodontal disease.

Abstract

Extracellular matrix metalloproteinase inducer (EMMPRIN) or CD147 is a transmembrane glycoprotein expressed by various cell types, including oral epithelial cells. Recent studies have brought evidence that EMMPRIN plays a role in periodontitis. In the present study, we investigated the effect of Porphyromonas gingivalis, a major pathogen in chronic periodontitis, on the shedding of membrane-anchored EMMPRIN and on the expression of the EMMPRIN gene by oral epithelial cells. A potential contribution of shed EMMPRIN to the inflammatory process of periodontitis was analyzed by evaluating the effect of recombinant EMMPRIN on cytokine and matrix metalloproteinase (MMP) secretion by human gingival fibroblasts. ELISA and immunofluorescence analyses revealed that P. gingivalis mediated the shedding of epithelial cell-surface EMMPRIN in a dose- and time-dependent manner. Cysteine proteinase (gingipain)-deficient P. gingivalis mutants were used to demonstrate that both Arg- and Lys-gingipain activities are involved in EMMPRIN shedding. Real-time PCR showed that P. gingivalis had no significant effect on the expression of the EMMPRIN gene in epithelial cells. Recombinant EMMPRIN induced the secretion of IL-6 and MMP-3 by gingival fibroblasts, a phenomenon that appears to involve mitogen activated protein kinases. The present study brought to light a new mechanism by which P. gingivalis can promote the inflammatory response during periodontitis.

• Authors: Mark Feldman, Vu Dang La, Telma Blanca Lombardo Bedran, Denise Madalena Palomari Spolidorio, Daniel Grenier
• Journal: Microbes and infection (Microbes Infect) Vol. 13 Issue 14-15 Pg. 1261-9 (Dec 2011) ISSN: 1769-714X [Electronic] France
• PMID: 21835259 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.

Chemical References

• Adhesins, Bacterial
• Gingipain Cysteine Endopeptidases
• Interleukin-6
• Recombinant Proteins
• Basigin
• Cysteine Endopeptidases
• Matrix Metalloproteinase 3

Topics

• Adhesins, Bacterial (genetics, immunology)
• Bacteroidaceae Infections (immunology, microbiology)
• Basigin (genetics, immunology, pharmacology)
• Cells, Cultured
• Cysteine Endopeptidases (deficiency, genetics, immunology)
• Enzyme-Linked Immunosorbent Assay
• Epithelial Cells (cytology, drug effects, immunology, microbiology)
• Fibroblasts (cytology, drug effects, immunology, microbiology)
• Gene Deletion
• Gingipain Cysteine Endopeptidases
• Gingiva (cytology, drug effects, immunology, microbiology)
• Humans
• Interleukin-6 (biosynthesis, immunology)
• Matrix Metalloproteinase 3 (biosynthesis, immunology)
• Periodontitis (immunology, microbiology)
• Porphyromonas gingivalis (genetics, immunology, metabolism)
• Real-Time Polymerase Chain Reaction
• Recombinant Proteins (genetics, immunology, pharmacology)