Abstract | PURPOSE: METHODS: Positron emission tomography (PET)/CT was performed on 14 patients with untreated localized primary prostate cancer 3 h post-injection of approximately 390 MBq of 18F-FAZA using forced diuresis to decrease radioactivity in the urinary bladder. Anatomical trans-pelvic coil and pre- and post-contrast 1.5 T MRI with endorectal coil were performed on the same day. Patients underwent radical prostatectomy and ex vivo 3 T MRI of the prostatectomy specimen within 14 days following in vivo imaging. Imaging results were verified by whole mount histopathology plus tissue microarray (TMA) immunohistochemical (IHC) analysis for carbonic anhydrase IX (CAIX) and hypoxia-inducible factor 1α (HIF-1α). Registration of in vivo imaging with histology was achieved using mutual information software and performing ex vivo MRI of the prostatectomy specimen and whole mount sectioning with block face photography as intermediate steps. RESULTS: Whole mount histology identified 43 tumor nodules, 19 of them larger than 1 ml as determined on coregistered volumes featuring 18F-FAZA, MRI, and histological 3-D image information. None of these lesions was found to be positive for CAIX or visualized by 18F-FAZA PET/CT while IHC for HIF-1α showed variable staining of tumor tissues. Accordingly, no correlation was found between 18F-FAZA uptake and Gleason scores. CONCLUSION:
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Authors | Rita Garcia-Parra, David Wood, Rajal B Shah, Javed Siddiqui, Hero Hussain, Hyunjin Park, Timothy Desmond, Charles Meyer, Morand Piert |
Journal | European journal of nuclear medicine and molecular imaging
(Eur J Nucl Med Mol Imaging)
Vol. 38
Issue 10
Pg. 1816-23
(Oct 2011)
ISSN: 1619-7089 [Electronic] Germany |
PMID | 21833840
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
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Chemical References |
- Nitroimidazoles
- fluoroazomycin arabinoside
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Topics |
- Aged
- Cell Hypoxia
- Humans
- Image Processing, Computer-Assisted
- Male
- Middle Aged
- Multimodal Imaging
(methods)
- Nitroimidazoles
- Positron-Emission Tomography
- Prostatic Neoplasms
(diagnostic imaging, pathology, surgery)
- Sensitivity and Specificity
- Tomography, X-Ray Computed
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