Hypoxia stimulates the production of the angiogenesis inhibitor 2-methoxyestradiol by swine granulosa cells.

Abstract

We previously demonstrated the presence of 2-methoxyestradiol (2-ME) in swine follicular fluid. Present study was aimed first of all to investigate if swine granulosa cell produce 2-ME; in addition, we tried to assess a potential effect of hypoxia in modulating 2-ME output. Finally, we explored the effect of 2-ME in an angiogenesis bioassay set up in our lab. Our data show that cultured granulosa cells are able to produce 2-ME; interestingly, the secretion of the hormone appeared to be stimulated by hypoxia. Angiogenesis bioassay points out that 2-ME displays an inhibitory effect on neovascularisation. Therefore our data suggest that 2-ME could be a local effector in determining the fine tuning responsible for follicle angiogenesis. These data deserve special attention since the ovary is a valuable experimental model in angiogenesis research.

• Authors: Giuseppina Basini, Francesca Grasselli, Simona Bussolati, Laura Baioni, Federica Bianchi, Marilena Musci, Maria Careri, Alessandro Mangia
• Journal: Steroids (Steroids) Vol. 76 Issue 13 Pg. 1433-6 (Dec 11 2011) ISSN: 1878-5867 [Electronic] United States
• PMID: 21827779 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 Elsevier Inc. All rights reserved.

Chemical References

• Angiogenesis Inhibitors
• Culture Media
• Estradiol
• 2-Methoxyestradiol
• Fibrin

Topics

• 2-Methoxyestradiol
• Angiogenesis Inhibitors (biosynthesis, pharmacology)
• Animals
• Aorta (cytology)
• Biological Assay
• Cell Hypoxia
• Cell Line
• Cell Proliferation (drug effects)
• Culture Media (metabolism)
• Endothelial Cells (cytology, drug effects)
• Estradiol (analogs & derivatives, biosynthesis, pharmacology)
• Female
• Fibrin (metabolism)
• Granulosa Cells (metabolism)
• Reproducibility of Results
• Swine