Abstract |
Chemical investigation of a new endophytic fungus, Mycosphaerella sp. nov. strain F2140, associated with the foliage of the plant Psychotria horizontalis (Rubiaceae) in Panama, resulted in the isolation of cercosporin (1) and a new cercosporin analog (3) as the major components. The structures of minor compounds in the extract were elucidated by detailed spectroscopic analysis as 2-(2-butyl)-6-ethyl-3-hydroxy-6-methylcyclohex-2-ene-1,5-dione (4), 3-(2-butyl)-6-ethyl-5-hydroxy-2-methoxy-6-methyl-cyclohex-2-enone (5), and an isomer of 5 (6). To study the influence of the hydroxy groups on the anti-parasitic activity of cercosporin, compound 1 was acetylated to obtain derivative 2. The isolated compounds 1- 6 were tested in vitro to determine their anti-parasitic activity against the causal agents of malaria (Plasmodium falciparum), leishmaniasis (Leishmania donovani), and Chagas disease (Trypanosoma cruzi). Cytotoxicity and potential anticancer activity of these compounds were evaluated using mammalian Vero cells and MCF7 cancer cell lines, respectively. Compounds 1 and 2 displayed high potency against L. donovani (IC50 0.46 and 0.64 microM), T. cruzi (IC50 1.08 and 0.78 microM), P. falciparum (IC50 1.03 and 2.99 microM), and MCF7 cancer cell lines (IC50 4.68 and 3.56 microM). Compounds 3-6 were not active in these assays at a concentration of 10 microg/mL.
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Authors | Eufemio Moreno, Titto Varughese, Carmenza Spadafora, A Elizabeth Arnold, Phyllis D Coley, Thomas A Kursar, William H Gerwick, Luis Cubilla-Rios |
Journal | Natural product communications
(Nat Prod Commun)
Vol. 6
Issue 6
Pg. 835-40
(Jun 2011)
ISSN: 1934-578X [Print] United States |
PMID | 21815421
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
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Chemical References |
- Antiprotozoal Agents
- Plant Extracts
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Topics |
- Animals
- Antiprotozoal Agents
(chemistry, pharmacology)
- Ascomycota
(chemistry)
- Chlorocebus aethiops
- Leishmania donovani
(drug effects)
- Molecular Structure
- Plant Extracts
(chemistry, pharmacology)
- Plasmodium falciparum
(drug effects)
- Trypanosoma cruzi
(drug effects)
- Vero Cells
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