Abstract | BACKGROUND AND AIM: METHODS: Murine CT-26 colon cancer cells stably transfected with MME were inoculated subcutaneously. Reverse-transcriptase polymerase chain reaction (RT-PCR), immunoblotting, and immunohistochemistry were used to explore the bFGF mRNA and protein expression. Immunohistochemical staining of CD34 was used to measure the microvessel density (MVD). RESULTS: bFGF mRNA levels in tumor tissues of CT-26-EGFP and nontransfected cells were respectively 2.7-fold (0.56 ± 0.063 vs. 0.21 ± 0.042) and 2.5-fold (0.53 ± 0.066 vs. 0.21 ± 0.042) higher than that in tumors of CT-26-EGFP-MME cells (p < 0.01). bFGF protein levels exhibited a similar trend. Tumors of CT-26-EGFP-MME cells demonstrated a lower microvessel density (9.35 ± 2.79) than control tumors of CT-26-EGFP cells (22.85 ± 3.80) and nontransfected cells (23.45 ± 4.49) (p < 0.001). CONCLUSIONS: We found that expression of MME inversely correlates with the expression of bFGF and tumor angiogenesis in a model of murine colon cancer. These data indicate that manipulation of MME expression could be a novel modality approach to colon cancer therapy.
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Authors | Zhangwei Xu, Hai Shi, Qiao Mei, Yuxian Shen, Jianming Xu |
Journal | Digestive diseases and sciences
(Dig Dis Sci)
Vol. 57
Issue 1
Pg. 85-91
(Jan 2012)
ISSN: 1573-2568 [Electronic] United States |
PMID | 21814804
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- RNA, Messenger
- Fibroblast Growth Factor 2
- Matrix Metalloproteinase 12
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Topics |
- Adenocarcinoma
(blood supply, metabolism, pathology)
- Animals
- Cell Line, Tumor
- Cell Proliferation
- Cell Transplantation
- Colonic Neoplasms
(blood supply, metabolism, pathology)
- Disease Models, Animal
- Female
- Fibroblast Growth Factor 2
(metabolism)
- Matrix Metalloproteinase 12
(genetics, metabolism)
- Mice
- Mice, Inbred BALB C
- Microvessels
(pathology)
- Neoplasm Transplantation
- Neovascularization, Pathologic
(metabolism, pathology)
- RNA, Messenger
(metabolism)
- Transfection
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