Heterogeneity and expression of the Plasmodium falciparum 5.8S ribosomal RNA genes.

Abstract

The number and expression of some of the large ribosomal RNA (rRNA) gene classes present in the human malaria parasite Plasmodium falciparum was determined. Southern blot analyses, using the 5.8S rRNA coding region as a marker, indicate that the P. falciparum genome contains at least 5 distinct subclasses of large rRNA genes. Dideoxy sequencing of the 5.8S rRNA domain and Northern blot analyses demonstrate that only one subclass is transcribed during the parasite's asexual erythrocytic life cycle.

• Authors: D Shippen-Lentz, T Afroze, A C Vezza
• Journal: Molecular and biochemical parasitology (Mol Biochem Parasitol) Vol. 38 Issue 1 Pg. 113-20 (Jan 01 1990) ISSN: 0166-6851 [Print] Netherlands
• PMID: 2181300 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• RNA, Ribosomal
• RNA, Ribosomal, 5.8S

Topics

• Animals
• Base Sequence
• Blotting, Northern
• Blotting, Southern
• Gene Expression Regulation
• Genomic Library
• Molecular Sequence Data
• Multigene Family (genetics)
• Plasmodium falciparum (genetics, growth & development)
• RNA, Ribosomal (genetics)
• RNA, Ribosomal, 5.8S (genetics)
• Sequence Homology, Nucleic Acid