Cell death-inducing effect of novel palladium(II) and platinum(II) complexes on non-small cell lung cancer cells in vitro.

Abstract

PURPOSE:

Treatment for lung cancer is still far from satisfying rates. Therefore, there is a need for novel anticancer agents. For this purpose, novel platinum and palladium complexes {[Pd(sac)(terpy)](sac)·4H(2)O (Complex 1), [Pt(sac)(terpy)](sac)·5H(2)O (Complex 2), [PdCl(terpy)](sac)·2H(2)O (Complex 3), [PtCl(terpy)](sac)·2H(2)O (Complex 4)} have been tested against three different non-small cell lung cancer cell lines (A549, H1299, PC-3).

METHODS:

Growth-inhibiting effects have been tested by the MTT and ATP viability assays. Apoptosis has been detected by the caspase-cleaved cytokeratin 18 (M30-antigen) assay. Necrosis has been detected by staining the cells with fluorescent dyes. Mitotic index has been calculated by counting the mitotic figures after staining with hematoxylin.

RESULTS:

The complex 3 exhibited significant anti-growth effects, and its anti-growth effect was more powerful than that of cisplatin that is a standard chemotherapeutic agent for this type of cancer. The complexes did not induce apoptosis, while necrosis clearly took place.

CONCLUSIONS:

Novel Pd(II) complex ([PdCl(terpy)](sac)·2H(2)O) seems to represent a potentially active drug against non-small cell lung cancer cell lines, and further studies in vivo are warranted.

• Authors: Engin Ulukaya, Ferda Ari, Konstantinos Dimas, Mehmet Sarimahmut, Emel Guney, Nikos Sakellaridis, Veysel T Yilmaz
• Journal: Journal of cancer research and clinical oncology (J Cancer Res Clin Oncol) Vol. 137 Issue 10 Pg. 1425-34 (Oct 2011) ISSN: 1432-1335 [Electronic] Germany
• PMID: 21809029 (Publication Type: Journal Article)

Chemical References

• Organometallic Compounds
• Organoplatinum Compounds
• Palladium
• Adenosine Triphosphate
• Cisplatin

Topics

• Adenosine Triphosphate (analysis)
• Apoptosis (drug effects)
• Carcinoma, Non-Small-Cell Lung (drug therapy, pathology)
• Cell Line, Tumor
• Cisplatin (pharmacology)
• Humans
• Lung Neoplasms (drug therapy, pathology)
• Mitosis (drug effects)
• Necrosis
• Organometallic Compounds (pharmacology)
• Organoplatinum Compounds (pharmacology)
• Palladium (pharmacology)