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Development of interdigitated arrays coated with functional polyaniline/MWCNT for electrochemical biodetection: application for human papilloma virus.

Abstract
In this study, polyaniline-multiwalled carbon nanotube film (PANi-MWCNT) has been polymerized on interdigitated platinum electrode arrays (IDA), fabricated by MEMS technology for the detection of human papillomavirus (HPV) infection, using immobilized peptide aptamers as affinity capture reagent. Label-free, electrochemical detection of the specific immune reaction between antigen peptide aptamer HPV-16-L1 (with a molecular weight of 1825 Da), the most common genotype in cytological normal women worldwide, and its specific antibody of HPV-16 (which is much bigger with molecular weight of ca. 150 kDa) on multifunctional PANi-MWCNT based arrays was reported. The most significant advantage of this technique consists of reagentless and multiple detection of antigen-antibody complex formation on well conducting IDA interface of PANi-MWCNT, without intermediate steps or any labeling reagents, as normally required in the previous works.
AuthorsLam Dai Tran, Dzung Tuan Nguyen, Binh Hai Nguyen, Quan Phuc Do, Huy Le Nguyen
JournalTalanta (Talanta) Vol. 85 Issue 3 Pg. 1560-5 (Sep 15 2011) ISSN: 1873-3573 [Electronic] Netherlands
PMID21807222 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2011 Elsevier B.V. All rights reserved.
Chemical References
  • Aniline Compounds
  • Antibodies, Viral
  • Antigens, Viral
  • Aptamers, Peptide
  • Capsid Proteins
  • Nanotubes, Carbon
  • Oncogene Proteins, Viral
  • polyaniline
  • L1 protein, Human papillomavirus type 16
Topics
  • Amino Acid Sequence
  • Aniline Compounds (chemistry)
  • Antibodies, Viral (immunology)
  • Antigens, Viral (immunology)
  • Aptamers, Peptide (analysis, chemistry, immunology)
  • Capsid Proteins (chemistry, genetics, immunology)
  • Electrochemical Techniques (instrumentation, methods)
  • Electrodes
  • Female
  • Human papillomavirus 16 (genetics, immunology)
  • Humans
  • Microscopy, Atomic Force
  • Microscopy, Electron, Scanning
  • Nanotubes, Carbon (chemistry, ultrastructure)
  • Oncogene Proteins, Viral (chemistry, genetics, immunology)
  • Papillomavirus Infections (diagnosis, immunology, virology)
  • Uterine Cervical Neoplasms (diagnosis, immunology, virology)

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