Abstract |
In this study, polyaniline-multiwalled carbon nanotube film (PANi-MWCNT) has been polymerized on interdigitated platinum electrode arrays (IDA), fabricated by MEMS technology for the detection of human papillomavirus (HPV) infection, using immobilized peptide aptamers as affinity capture reagent. Label-free, electrochemical detection of the specific immune reaction between antigen peptide aptamer HPV-16-L1 (with a molecular weight of 1825 Da), the most common genotype in cytological normal women worldwide, and its specific antibody of HPV-16 (which is much bigger with molecular weight of ca. 150 kDa) on multifunctional PANi-MWCNT based arrays was reported. The most significant advantage of this technique consists of reagentless and multiple detection of antigen-antibody complex formation on well conducting IDA interface of PANi-MWCNT, without intermediate steps or any labeling reagents, as normally required in the previous works.
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Authors | Lam Dai Tran, Dzung Tuan Nguyen, Binh Hai Nguyen, Quan Phuc Do, Huy Le Nguyen |
Journal | Talanta
(Talanta)
Vol. 85
Issue 3
Pg. 1560-5
(Sep 15 2011)
ISSN: 1873-3573 [Electronic] Netherlands |
PMID | 21807222
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2011 Elsevier B.V. All rights reserved. |
Chemical References |
- Aniline Compounds
- Antibodies, Viral
- Antigens, Viral
- Aptamers, Peptide
- Capsid Proteins
- Nanotubes, Carbon
- Oncogene Proteins, Viral
- polyaniline
- L1 protein, Human papillomavirus type 16
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Topics |
- Amino Acid Sequence
- Aniline Compounds
(chemistry)
- Antibodies, Viral
(immunology)
- Antigens, Viral
(immunology)
- Aptamers, Peptide
(analysis, chemistry, immunology)
- Capsid Proteins
(chemistry, genetics, immunology)
- Electrochemical Techniques
(instrumentation, methods)
- Electrodes
- Female
- Human papillomavirus 16
(genetics, immunology)
- Humans
- Microscopy, Atomic Force
- Microscopy, Electron, Scanning
- Nanotubes, Carbon
(chemistry, ultrastructure)
- Oncogene Proteins, Viral
(chemistry, genetics, immunology)
- Papillomavirus Infections
(diagnosis, immunology, virology)
- Uterine Cervical Neoplasms
(diagnosis, immunology, virology)
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