Does formaldehyde induce aneuploidy?

Abstract

Formaldehyde (FA) was tested for a potential aneugenic activity in mammalian cells. We employed tests to discriminate between aneugenic and clastogenic effects in accordance with international guidelines for genotoxicity testing. The cytokinesis-block micronucleus test (CBMNT) in combination with fluorescence in situ hybridisation (FISH) with a pan-centromeric probe was performed with cultured human lymphocytes and the human A549 lung cell line. FA induced micronuclei (MN) in binuclear cells of both cell types under standard in vitro test conditions following the OECD guideline 487. FISH analysis revealed that the vast majority of induced MN were centromere negative, thus indicating a clastogenic effect. A similar result was obtained for MN induced by γ-irradiation, whereas the typical aneugens colcemid (COL) and vincristine (VCR) predominantly induced centromere-positive MN. Furthermore, COL and VCR clearly enhanced the MN frequency in mononuclear lymphocytes in the CBMNT, whereas such an effect was not observed for γ-irradiation and FA. In experiments with the Chinese hamster V79 cell line, the aneugens COL and VCR clearly increased the frequency of tetraploid second division metaphases, whereas FA did not cause such an effect. Altogether, our results confirm the clastogenicity of FA in cultured mammalian cells but exclude a significant aneugenic activity.

• Authors: Günter Speit, Stefanie Kühner, Regina Linsenmeyer, Petra Schütz
• Journal: Mutagenesis (Mutagenesis) Vol. 26 Issue 6 Pg. 805-11 (Nov 2011) ISSN: 1464-3804 [Electronic] England
• PMID: 21804075 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Formaldehyde
• Vincristine
• Bromodeoxyuridine
• Demecolcine

Topics

• Aneuploidy
• Animals
• Bromodeoxyuridine (metabolism)
• Cell Line
• Cricetinae
• Demecolcine (pharmacology)
• Formaldehyde (toxicity)
• Humans
• In Situ Hybridization, Fluorescence
• Lymphocytes (drug effects, metabolism)
• Micronuclei, Chromosome-Defective (radiation effects)
• Polyploidy
• Radiation, Ionizing
• Sister Chromatid Exchange (drug effects, radiation effects)
• Vincristine (pharmacology)