Abstract | BACKGROUND: METHODOLOGY/PRINCIPAL FINDINGS: Here we examined the effect of ESPL1 heterozygosity using a hypomorphic mouse model that has reduced germline Separase activity. We report that while ESPL1 mutant (ESPL1 (+/hyp)) mice have a normal phenotype, in the absence of p53, these mice develop spontaneous T- and B-cell lymphomas, and leukemia with a significantly shortened latency as compared to p53 null mice. The ESPL1 hypomorphic, p53 heterozygous transgenic mice (ESPL1(+/hyp), p53(+/-)) also show a significantly reduced life span with an altered tumor spectrum of carcinomas and sarcomas compared to p53(+/-) mice alone. Furthermore, ESPL1(+/hyp), p53(-/-) mice display significantly higher levels of genetic instability and aneuploidy in normal cells, as indicated by the abnormal metaphase counts and SKY analysis of primary splenocytes. CONCLUSIONS/SIGNIFICANCE: Our results indicate that reduced levels of Separase act synergistically with loss of p53 in the initiation and progression of B- and T- cell lymphomas, which is aided by increased chromosomal missegregation and accumulation of genomic instability. ESPL1(+/hyp), p53(-/-) mice provide a new animal model for mechanistic study of aggressive lymphoma and also for preclinical evaluation of new agents for its therapy.
|
Authors | Malini Mukherjee, Gouqing Ge, Nenggang Zhang, Eryong Huang, Lanelle V Nakamura, Marissa Minor, Viacheslav Fofanov, Pullivarthi H Rao, Alan Herron, Debananda Pati |
Journal | PloS one
(PLoS One)
Vol. 6
Issue 7
Pg. e22167
( 2011)
ISSN: 1932-6203 [Electronic] United States |
PMID | 21799785
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
|
Chemical References |
- Cell Cycle Proteins
- Tumor Suppressor Protein p53
- Endopeptidases
- ESPL1 protein, human
- Separase
|
Topics |
- Aneuploidy
- Animals
- Bone Marrow
(metabolism, pathology)
- Cell Cycle Proteins
(deficiency, genetics, metabolism)
- Cell Proliferation
- DNA Damage
(genetics)
- Disease Progression
- Endopeptidases
(deficiency, genetics, metabolism)
- Female
- Humans
- Leukemia
(enzymology, genetics, pathology)
- Longevity
(genetics)
- Lymphoma, B-Cell
(enzymology, genetics, pathology)
- Lymphoma, T-Cell
(enzymology, genetics, pathology)
- Mice
- Neoplasm Metastasis
- Phenotype
- Separase
- Thymus Neoplasms
(enzymology, genetics, pathology)
- Tumor Suppressor Protein p53
(deficiency, genetics, metabolism)
|