Inhibitory effects of interferon-gamma plasmid DNA on DMBA-TPA induced mouse skin carcinogenesis.

Abstract

Interferon-gamma (IFN-γ) exhibits biological activities that are considered to have important roles in tumor suppression. Therefore, the IFN-γ gene is a potential candidate for in vivo cytokine gene therapy against skin cancer. The present study evaluated the efficacy of a hydrodynamics-based IFN-γ gene transfection for skin cancer treatment, in which the plasmid DNA encoding IFN-γ was administered into the tail vein of mice following 7,12-dimethylbenz[a]anthracene and 12-O-tetradecanoylphorbol-13-acetate-induced skin carcinogenesis. Serum levels of IFN-γ were substantially elevated without liver toxicity. The mice injected with IFN-γ plasmid DNA displayed a marked reduction in papilloma numbers, suppressed proliferation of epidermal cells and induction of caspase-3-mediated apoptosis. These results suggest that the hydrodynamics-based transfection of IFN-γ plasmid DNA is a convenient and efficient means of skin cancer gene therapy.

• Authors: J-H Ko, B-G Jung, Y-S Park, B-J Lee
• Journal: Cancer gene therapy (Cancer Gene Ther) Vol. 18 Issue 9 Pg. 646-54 (Sep 2011) ISSN: 1476-5500 [Electronic] England
• PMID: 21799530 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• 9,10-Dimethyl-1,2-benzanthracene
• Interferon-gamma
• Caspase 3
• Tetradecanoylphorbol Acetate

Topics

• 9,10-Dimethyl-1,2-benzanthracene (toxicity)
• Animals
• Apoptosis (genetics)
• Caspase 3 (genetics, metabolism)
• Cell Proliferation
• Female
• Genetic Therapy
• Interferon-gamma (genetics, metabolism)
• Mice
• Mice, Inbred BALB C
• Plasmids
• Skin Neoplasms (chemically induced, therapy)
• Tetradecanoylphorbol Acetate (toxicity)